It is often desirable to evaluate the ability of cells to move in an unrestricted manner in multiple directions without chemical gradients. By combining the standard radial migration assay with injection-molded gaskets and a rigid fixture, we have developed a highly reliable and sensitive method for observing and measuring radial cell migration. This method is adapted for use on high-throughput automated imaging systems. The use of injection-molded gaskets enables low-cost replacement of cell-wetted components. Moreover, the design enables secondary placement of attractants and co-cultures. This device and its enhanced throughput permit the use of therapeutic screening to evaluate phenotypic responses, for example, cancer cell migration response due to drugs or chemical signals. This approach is orthogonal to other 2D cell migration applications, such as scratch wound assays, although here we offer a noninvasive, enhanced-throughput device, which currently is not commercially available but is easily constructed. The proposed device is a systematic, reliable, rapid application to monitor phenotypic responses to chemotherapeutic screens, genetic alterations (e.g., RNAi and CRISPR), supplemental regimens, and other approaches, offering a reliable methodology to survey unbiased and noninvasive cell migration.

通常需要评估细胞在没有化学梯度的情况下以不受限制的方式在多个方向上移动的能力。通过将标准径向迁移测定法与注塑成型垫圈和刚性夹具相结合，我们开发了一种高度可靠和灵敏的方法来观察和测量径向细胞迁移。该方法适用于高通量自动成像系统。使用注塑成型垫圈可以低成本更换电池润湿组件。此外，该设计能够二次放置引诱剂和共培养物。该设备及其增强的吞吐量允许使用治疗筛选来评估表型反应，例如，由于药物或化学信号引起的癌细胞迁移反应。这种方法与其他二维细胞迁移应用正交，例如划痕检测，尽管我们在这里提供了一种无创的、增强的通量装置，该装置目前尚未商业化，但很容易构建。所提出的装置是一种系统、可靠、快速的应用程序，用于监测对化疗筛选、基因改变（例如 RNAi 和 CRISPR）、补充方案和其他方法的表型反应，为调查无偏见和无创细胞迁移提供了一种可靠的方法。