Interferon-induced transmembrane proteins (IFITMs) are transmembrane proteins induced by interferon that can provide broad-spectrum antiviral activities. However, there are few reports on the antiviral activity of monkey-derived IFITMs. In this study, the IFITM1 and IFITM3 genes of African green monkey (AGM) were cloned and overexpressed in Vero cells, followed by infection with mouse norovirus (MNV) and severe fever with thrombocytopenia syndrome virus (SFTSV). The results showed that monkey IFITM1 and IFITM3 can be stably overexpressed in Vero cells. Both IFITM1 and IFITM3 from AGM could effectively restrict infection by SFTSV, and the viral inhibition rate of IFITM3 was more obvious compared with IFITM1. However, both monkey IFITM1 and IFITM3 had no significant effect on the replication of MNV. These results indicate that different IFITMs have different functions, which may be related to the structure of the host IFITMs and the types of pathogens.

中文翻译：

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非洲绿猴的 IFITMs 可以抑制 SFTSV 的复制，但不能抑制 MNV 体外

干扰素诱导的跨膜蛋白 (IFITM) 是由干扰素诱导的跨膜蛋白，可提供广谱抗病毒活性。然而，关于猴源性 IFITMs 的抗病毒活性的报道很少。在本研究中，IFITM1和IFITM3克隆非洲绿猴（AGM）基因并在Vero细胞中过表达，随后感染小鼠诺如病毒（MNV）和发热伴血小板减少综合征病毒（SFTSV）。结果表明，猴IFITM1和IFITM3可以在Vero细胞中稳定过表达。AGM的IFITM1和IFITM3均能有效抑制SFTSV的感染，IFITM3的病毒抑制率较IFITM1更明显。然而，猴IFITM1和IFITM3对MNV的复制没有显着影响。这些结果表明不同的 IFITMs 具有不同的功能，这可能与宿主 IFITMs 的结构和病原体的类型有关。