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Nanocrystals Continuously Releasing Nitric Oxide: Promoting Cell Migration and Increasing Cell Survival against Oxidative Stress
Chemistry of Materials ( IF 7.2 ) Pub Date : 2020-11-12 , DOI: 10.1021/acs.chemmater.0c03800 Hyejoong Jeong 1 , Daheui Choi 1 , Junjira Tanum 1 , Yoogyeong Oh 1 , Kyungtae Park 1 , Jinkee Hong 1
Chemistry of Materials ( IF 7.2 ) Pub Date : 2020-11-12 , DOI: 10.1021/acs.chemmater.0c03800 Hyejoong Jeong 1 , Daheui Choi 1 , Junjira Tanum 1 , Yoogyeong Oh 1 , Kyungtae Park 1 , Jinkee Hong 1
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Antioxidants offer a potential cure for certain diseases caused by reactive oxygen species (ROS); unfortunately, conventional antioxidants have poor long-term targeting and bioavailability. Here, we report NO-releasing crystals (NOCs) to achieve direct ROS scavenging as well as enhance cell survival, based on their long-term NO supply. Despite the controversial functions of NO, in order to achieve the goal, NOCs can supply a constant level of NO over 4 days, owing to the thermally stable form of C-diazeniumdiolate-based NO donor. NOCs are controllable for their size, aspect ratio, and NO loading by the crystallization temperature; the nanosized NOCs enhanced cytocompatibility and cell migration owing to their excellent distribution and supply of NO at physiological levels. In addition, NOCs exhibited efficient and continuous radical scavenging compared to ascorbic acid (AA) as a function of the NO release amount. An in vitro test on the antioxidative activity of NO was investigated via two methods: pretreatment of antioxidants prior to oxidative stress and antioxidant treatment in the presence of the oxidants. It was found that 10–20 μM of NO from NOC was optimal for cell protection under oxidative stress. These findings demonstrate the controlled NO system and potential utility of NO as an effective antioxidation tool for treating diseases induced by ROS overproduction.
中文翻译：
纳米晶体连续释放一氧化氮：促进细胞迁移，并提高细胞存活率，抵抗氧化应激。
抗氧化剂为某些由活性氧（ROS）引起的疾病提供了潜在的治疗方法。不幸的是，常规的抗氧化剂具有很差的长期靶向性和生物利用度。在这里，我们报告了基于NO长期提供的NO释放晶体（NOC），以实现直接ROS清除并提高细胞存活率。尽管存在有争议的NO功能，但为了实现这一目标，由于C的热稳定形式，NOC可以在4天内提供恒定水平的NO。-基于二醇二氮烯鎓的NO供体。NOC的尺寸，长宽比和NO的加载量受结晶温度的控制。纳米级NOC由于其在生理水平上的出色的NO分布和供应而增强了细胞相容性和细胞迁移。此外，与抗坏血酸（AA）相比，NOC表现出有效且连续的自由基清除能力，这是NO释放量的函数。一种在体外通过两种方法对NO的抗氧化活性进行了测试：在氧化应激之前对抗氧化剂进行预处理以及在氧化剂存在下进行抗氧化剂处理。已发现，来自NOC的10–20μMNO最适合在氧化应激下保护细胞。这些发现证明了受控的NO系统和NO作为治疗ROS过量生产引起的疾病的有效抗氧化工具的潜在用途。
更新日期：2020-11-25
中文翻译：
纳米晶体连续释放一氧化氮：促进细胞迁移，并提高细胞存活率，抵抗氧化应激。
抗氧化剂为某些由活性氧（ROS）引起的疾病提供了潜在的治疗方法。不幸的是，常规的抗氧化剂具有很差的长期靶向性和生物利用度。在这里，我们报告了基于NO长期提供的NO释放晶体（NOC），以实现直接ROS清除并提高细胞存活率。尽管存在有争议的NO功能，但为了实现这一目标，由于C的热稳定形式，NOC可以在4天内提供恒定水平的NO。-基于二醇二氮烯鎓的NO供体。NOC的尺寸，长宽比和NO的加载量受结晶温度的控制。纳米级NOC由于其在生理水平上的出色的NO分布和供应而增强了细胞相容性和细胞迁移。此外，与抗坏血酸（AA）相比，NOC表现出有效且连续的自由基清除能力，这是NO释放量的函数。一种在体外通过两种方法对NO的抗氧化活性进行了测试：在氧化应激之前对抗氧化剂进行预处理以及在氧化剂存在下进行抗氧化剂处理。已发现，来自NOC的10–20μMNO最适合在氧化应激下保护细胞。这些发现证明了受控的NO系统和NO作为治疗ROS过量生产引起的疾病的有效抗氧化工具的潜在用途。
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