Recycling of Black Liquor for Treating Sugarcane Bagasse at Low Temperature to Attain High Ethanol Production without Washing Step,ACS Sustainable Chemistry & Engineering

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Recycling of Black Liquor for Treating Sugarcane Bagasse at Low Temperature to Attain High Ethanol Production without Washing Step
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-11-12 , DOI: 10.1021/acssuschemeng.0c05763
Qingfeng Wang _{1,

2} , Wen Wang ₁ , Zahoor ₁ , Xuesong Tan ₁ , Xinshu Zhuang ₁ , Changlin Miao ₁ , Ying Guo ₁ , Xiaoyan Chen ₁ , Qiang Yu ₁ , Zhenhong Yuan _{1,

3}

Affiliation

Alkaline pretreatment has been commonly used for bioconversion of lignocellulose into fuel ethanol due to its gentle condition. However, the large quantity of water consumption and wastewater generation from the pretreatment and washing steps have been two bottleneck problems for the practical application of alkaline pretreatment. In order to save water consumption and reduce wastewater generation, black liquor (BL) generated in the sodium hydroxide (NaOH) pretreatment process was recycled for pretreating sugarcane bagasse (SCB), and the BL-NaOH-treated SCB without being washed was directly enzymatically hydrolyzed and fermented. After 120-h fed-batch enzymatic hydrolysis with the addition of 20 FPU cellulase/g cellulose and 2.51 μL Tween80/mL hydrolytic slurry, the maximum glucose concentration and enzymatic hydrolysis efficiency achieved 99.95 g/L and 71.02%, respectively. After 36-h fermentation with the domesticated Saccharomyces cerevisiae, the ethanol production reached 44.53 g/L, which amounted to 87.35% of theoretical ethanol yield. Compared with other water-saving and wastewater-reducing alkaline pretreatments, this technology saved more than 20% water consumption and reduced over 20% wastewater discharge. It provides a promising applied technology for cellulosic ethanol production.

中文翻译：

循环利用黑液在低温下处理甘蔗渣以达到高乙醇产量而无需洗涤步骤

由于其条件温和，碱性预处理已普遍用于将木质纤维素生物转化为燃料乙醇。然而，预处理和洗涤步骤中大量的水消耗和废水产生已经成为碱性预处理的实际应用中的两个瓶颈问题。为了节约用水并减少废水的产生，将氢氧化钠（NaOH）预处理过程中产生的黑液（BL）进行循环以对甘蔗渣（SCB）进行预处理，并且将未经洗涤的BL-NaOH处理过的SCB直接进行酶促处理水解和发酵。在添加20 FPU纤维素酶/ g纤维素和2.51μLTween80 / mL水解浆液进行120小时分批补料分批酶解后，最大葡萄糖浓度和酶促水解效率达到99。分别为95 g / L和71.02％。经过驯化36小时发酵酿酒酵母，乙醇产量达到44.53 g / L，占理论乙醇产量的87.35％。与其他节水和减少废水的碱性预处理相比，该技术节省了20％以上的用水量，并减少了20％以上的废水排放量。它为纤维素乙醇生产提供了有希望的应用技术。

更新日期：2020-11-23

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