Asian citrus psyllid, Diaphorina citri (Kuwayama), preferentially orient toward citrus hosts infected with the phytopathogenic bacterium, Candidatus liberibacter asiaticus (CLas) the agent of citrus greening (Huanglongbing, HLB), compared to uninfected counterparts. We investigated whether this preference for the odors of infected plants could be useful for the development of an attract-and-kill (AK) device for D. citri. Twenty-nine blends of volatile organic compounds derived from the odor of citrus infected with CLas were tested in laboratory olfactometer tests, and two blends were also assessed under field conditions. A seven component blend of tricosane: geranial: methyl salicylate: geranyl acetone: linalool: phenylacetaldehyde: (E)-β-ocimene in a 0.40: 0.06: 0.08: 0.29: 0.08: 0.06: 0.03 ratio released from a proprietary slow-release matrix attracted twice more D. citri to yellow sticky traps compared with blank control traps. The attractive blend was subsequently co-formulated with spinosad insecticide into a slow-release matrix to create a prototype AK formulation against D. citri. This formulation effectively reduced the population density of D. citri up to 84% as measured with tap counts when deployed at a density of eight 2.5 g dollops per tree as compared with untreated controls in small plot field trials conducted in citrus orchards. Psyllid populations were not statistically affected at a deployment rate of four dollops per tree. Our results indicate that an AK formulation incorporating spinosad and a volatile blend signature of citrus greening into a slow-release matrix may be useful to suppress D. citri populations.

中文翻译：

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利用黄龙病感染柑橘的挥发性特征开发亚洲柑橘木虱（半翅目：丽蝇科）诱杀装置的研究进展

亚洲柑橘木虱，柑橘木虱（桑山），优先朝向感染了植物病原细菌，柑橘主机定向暂定liberibacter胭脂（Ç LAS）柑橘青果（黄龙病，HLB）的试剂，相对于未感染的对应物。我们调查了这种对被感染植物气味的偏爱是否可用于开发柠檬果蝇的诱杀（AK）装置。二十九种挥发性有机化合物的混合物，这些混合物来自于感染C的柑橘气味在实验室嗅觉仪测试中对Las进行了测试，并且还在野外条件下评估了两种混合物。从专有的缓释基质中以0.40：0.06：0.08：0.29：0.08：0.06：0.03的比例释放出的三组分组成的二十三烷：香叶醛：水杨酸甲酯：香叶基丙酮：芳樟醇：苯乙醛：（E）-β-烯丙烯与空白对照诱集剂相比，柠檬酸诱集剂比黄色诱集剂多两倍。随后将诱人的掺合物与多杀菌素杀虫剂一起配制到缓释基质中，以制得对抗柠檬衣藻的原型AK制剂。这种配方有效地降低了柠檬果蝇的种群密度在柑橘园中进行的小块田间试验中，与未经处理的对照相比，当以每棵树八个2.5 g doll的密度展开时，用抽头计数测得的结果高达84％。在每棵树四个doll的部署率下，木虱种群没有受到统计影响。我们的结果表明，将多杀菌素和柑桔绿化的挥发性混合物特征掺入到缓释基质中的AK制剂可能对抑制柠檬果蝇种群有用。