The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.

中文翻译：

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从尿源性干细胞产生的 3-D 人肾小管类器官用于肾毒性筛查

迫切需要开发基于人类细胞的系统来替代啮齿动物或细胞的二维培养来研究肾毒性。在肾细胞外基质诱导后，人尿源性干细胞在三维 (3-D) 培养中分化为肾小管上皮细胞。响应丙酮和顺铂，3-D 类器官中 CYP2E1 和 KIM-1 的水平显着增加。这种 3-D 培养系统为肾毒性筛查和研究提供了一种替代工具。