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Direct-current electric field effect on the viability of HeLa cell line
Electromagnetic Biology and Medicine ( IF 1.6 ) Pub Date : 2020-11-12 , DOI: 10.1080/15368378.2020.1846193
Afdhal Muttaqin 1 , Sumaryati Syukur 1 , Yulkifli Yulkifli 1 , Tofrizal Alimuddin 1
Affiliation  

Electric fields affect cell life, cancer cells are not spared. Research on the effectiveness of electric fields on the life of cancer cells is carried out using HeLa cells as target cells receiving an electric-field treatment for 24 h. This study is a laboratory experimental study of the viability of cancer cells (HeLa cells), measured by employing the MTT assay method. Experiments are carried out by administering a low direct-current electric field utilizing a couple of aluminum electrode plates on the HeLa cell line, planted in a micro-culture plate with voltages ranging from 46.67 V/m to 600.00 V/m. The dcEF was found to have a profound inhibitory effect on HeLa cell line viability, except at dcEF 93.33 V/m which shows anomalies, in the form of increased viability over control viability (115%). The mortality index reaches almost 100% when induced by dcEF>300.00 V/m. It was observed that the HeLa cell size is larger after dcEF induction was applied.

中文翻译:


直流电场对HeLa细胞系活力的影响



电场影响细胞生命,癌细胞也不能幸免。以HeLa细胞为靶细胞,接受电场处理24小时,研究电场对癌细胞生命的有效性。本研究是对癌细胞(HeLa 细胞)活力的实验室实验研究,采用 MTT 测定方法进行测量。实验是通过在 HeLa 细胞系上使用几个铝电极板施加低直流电场来进行的,该细胞系种植在微培养板中,电压范围为 46.67 V/m 至 600.00 V/m。发现 dcEF 对 HeLa 细胞系活力具有深远的抑制作用,但 dcEF 93.33 V/m 时除外,其表现出异常,其形式为活力高于对照活力 (115%)。当dcEF>300.00 V/m诱导时,死亡率指数几乎达到100%。观察到应用 dcEF 诱导后 HeLa 细胞尺寸更大。
更新日期:2020-11-12
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