Abstract

Syzygium cumini, owing to higher bioactive constituents, its parts principally kernels are used for the antidiabetic purpose since the olden days. The current manuscript illustrated batch extraction of phenolic compounds from S. cumini using a stirred extractor. The yields 0.61 mg/g, 35.9 mg/g, 79.89 mg GAE/g, and 7.29 mg CE/g of catechin, gallic acid, TPC and TFC, respectively, were obtained in 105 min. at 1:20 SCKP to water, 50 ± 2 °C temperature, 4 pH, at 250 rpm and 106 µm particle size of SCKP. In vitro evaluation of the antioxidant and antidiabetic potential of the obtained aqueous extract was carried out by DPPH, α-amylase, and α-glucosidase inhibitory assays. The IC₅₀ values of SCKP aqueous extract obtained were 12.97, 9.03, and 7.13 µg/mL for DPPH scavenging, inhibition of α-amylase, and α-glucosidase, respectively. The cost required to extract 1 kg of catechin, gallic acid, TPC, and TFC was Rs 6691.6, 113.7, 51.1, and 559.93/-, respectively. Stirred batch extraction technique manifests traditional but simple, ecofriendly, and efficient compared to other traditional techniques. The output of this research bestows support to utilize the SCKP stirred batch extract as a promising source of antioxidant and antidiabetic compounds in ayurvedic formulations.

摘要

Syzygium cumini，由于具有较高的生物活性成分，其部分主要是仁自古以来就被用于抗糖尿病目的。当前手稿说明了使用搅拌提取器从小茴香中批量提取酚类化合物。在 105 分钟内，儿茶素、没食子酸、TPC 和 TFC 的产量分别为 0.61 mg/g、35.9 mg/g、79.89 mg GAE/g 和 7.29 mg CE/g。SCKP 与水的比例为 1:20，温度为 50 ± 2 °C，pH 值为 4，转速为 250 rpm，SCKP 粒径为 106 µm。通过 DPPH、α-淀粉酶和 α-葡萄糖苷酶抑制试验对所得水提取物的抗氧化和抗糖尿病潜力进行体外评价。IC ₅₀所获得的 SCKP 水提取物的 DPPH 清除、α-淀粉酶和α-葡萄糖苷酶抑制值分别为 12.97、9.03 和 7.13 µg/mL。提取 1 kg 儿茶素、没食子酸、TPC 和 TFC 所需的成本分别为 6691.6、113.7、51.1 和 559.93 卢比/-。搅拌分批萃取技术与其他传统技术相比，具有传统但简单、环保、高效的特点。这项研究的结果支持将 SCKP 搅拌批次提取物用作阿育吠陀配方中抗氧化剂和抗糖尿病化合物的有希望的来源。