Abstract

Forsythia saxatilis (Nakai) Nakai is an endemic species in Korea. We present the second complete chloroplast genome sequence of F. saxatilis showing that the chloroplast genome is 156,376 bp in length containing four subregions: a large single-copy (LSC) region of 87,097 bp, a small single-copy (SSC) region of 17,859 bp, and a pair of inverted repeat regions (IRs) of 25,710 bp. The genome contains 131 genes including 88 protein-coding genes, 35 tRNAs, and eight rRNAs. We found 40 base differences in 17 sites between our chloroplast genome and the previously reported chloroplast genome sequences of F. saxatilis. All of the differences were found in a region of 832 bp in the LSC region except for one site, which indicated potential sequencing errors in building the previously reported genome because of little substitutions in the homologous region of the other Forsythia species and even of Abeliophyllum distichum, a sister to Forsythia. The new chloroplast genomes of F. saxatilis and those of F. x intermedia and F. viridissima are identical, which suggests that F. saxatilis is maternally closely related to the other two species.

摘要

连翘（Nakai）Nakai是韩国的特有种。我们提出的第二完整叶绿体基因组序列F.鲈表示叶绿体基因组是156376 bp的含有四个子区域长度：87097碱基对，的17859小单拷贝（SSC）区域的大单拷贝（LSC）区域bp，以及一对25,710 bp的反向重复区域（IR）。基因组包含131个基因，包括88个蛋白质编码基因，35个tRNA和8个rRNA。我们发现40间碱基的差异在我们的叶绿体基因组与先前报道的叶绿体基因组序列之间的17点F.黑眉。除一个位点外，所有差异均在LSC区域的832 bp区域中发现，这表明在构建先前报道的基因组时可能存在测序错误，原因是其他连翘属植物甚至是拟南芥的同源区域中几乎没有取代。是连翘的妹妹。的新的基因组叶绿体F.鲈和那些的F. X中间和F. viridissima是相同的，这表明F.鲈是母系密切相关的其它两个物种。