Abstract

The two complete mitochondrial genomes were sequenced from the freshwater monogonont rotifer Brachionus angularis. The mitochondrial genome sequences were 10,764 bp (mitochondrial DNA I) and 12,238 bp (mitochondrial DNA II) in size, respectively. The gene structure and its orientation of 12 protein-coding genes (PCGs) of complete mitochondrial genomes of B. angularis was identical to those shown in other marine rotifers and the freshwater rotifer Brachionus rubens, but was different from the freshwater rotifer Brachionus calyciflorus. Of 12 PCGs, one gene (ND5) had incomplete stop codon. Furthermore, the start codon for CO1, ND4L, ND5, and CO2 was GTG, while the start codon for ND3 and other PCGs was ATA and ATG, respectively. The base composition of 12 PCGs in B. angularis mitogenome showed 20.4% for A, 47.3% for T, 17.5% for C, and 14.8% for G, respectively. The mitochondrial genome A + T base composition (67.7%) of 12 PCGs was higher than G + C (32.3%), while the complete mitochondrial genome A + T base composition (66.3%) was higher than G + C (33.7%).

摘要

这两个完整的线粒体基因组是从淡水轮虫轮虫Brachionus angularis测序的。线粒体基因组序列的大小分别为10,764 bp（线粒体DNA I）和12,238 bp（线粒体DNA II）。角衣芽孢杆菌完整线粒体基因组的12个蛋白质编码基因（PCG）的基因结构和方向与其他海洋轮虫和淡水轮虫Brachionus rubens中显示的相同，但不同于淡水轮虫Brachionus calyciflorus。在12个PCG中，一个基因（ND5）的终止密码子不完整。此外，CO1，ND4L，ND5的起始密码子，CO2为GTG，而ND3和其他PCG的起始密码分别为ATA和ATG。角芽孢杆菌有丝分裂基因组中的12种PCG的基本组成分别为：A为20.4％，T为47.3％，C为17.5％，G为14.8％。12个PCG的线粒体基因组A + T基础组成（67.7％）高于G + C（32.3％），而完整的线粒体基因组A + T基础组成（66.3％）高于G + C（33.7％） 。