Aging is a fundamental and fascinating process. Anti‐aging research tried to find the mysteries about the human lifespan. To investigate the longevity‐extending role of caffeic acid phenethylester (CAPE) and reveal the possible regulation mechanism, the long‐term cultivation under well‐defined environments, real‐time monitoring, and live imaging is highly desired. In this paper, a well‐designed microfluidic device was proposed to analyze the anti‐aging effect of CAPE in Caenorhabditis elegans. With the combined use of multiple functional units, including micro‐pillar, worm responder, a branching network of distribution channels, and microchambers, the longitudinal measurements of the exact number of worms throughout the whole lifespans is possible. Meanwhile, the brief cooling function of temperature‐controllable system can achieve temporary and repeated immobilization of nematodes for fluorescence imaging. Our research data showed that CAPE can increase the survival of worms under normal and stress condition, including heat stress and paraquat‐induced oxidative stress. The further studies revealed the anti‐aging mechanism of CAPE. This proposed strategy and device would be a useful platform to facilitate future anti‐aging studies and the finding of new lead compounds.

中文翻译：

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用于评估咖啡酸苯乙酯对秀丽隐杆线虫抗衰老作用的集成微流体

衰老是一个基本而迷人的过程。抗衰老研究试图揭开人类寿命的奥秘。为了研究咖啡酸苯乙酯（CAPE）的长寿作用并揭示其可能的调节机制，非常需要在明确定义的环境下长期培养、实时监测和实时成像。在本文中，提出了一种设计良好的微流体装置来分析 CAPE 对秀丽隐杆线虫的抗衰老作用. 通过结合使用多个功能单元，包括微柱、蠕虫响应器、分配通道的分支网络和微室，可以纵向测量整个生命周期中蠕虫的确切数量。同时，温控系统的短暂冷却功能可以实现线虫的临时和重复固定以进行荧光成像。我们的研究数据表明，CAPE 可以提高蠕虫在正常和应激条件下的存活率，包括热应激和百草枯诱导的氧化应激。进一步的研究揭示了CAPE的抗衰老机制。这一提议的策略和设备将成为促进未来抗衰老研究和寻找新先导化合物的有用平台。