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Recovering the osteoblastic differentiation potential of mesenchymal stem cells derived from diabetic rats by photobiomodulation therapy
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-11-12 , DOI: 10.1002/jbio.202000393 Natália Pieretti Bueno 1 , Isabella Nunes Copete 1 , Helena Bacha Lopes 2 , Praveen R Arany 3 , Márcia Martins Marques 1, 4 , Emanuela Prado Ferraz 1
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-11-12 , DOI: 10.1002/jbio.202000393 Natália Pieretti Bueno 1 , Isabella Nunes Copete 1 , Helena Bacha Lopes 2 , Praveen R Arany 3 , Márcia Martins Marques 1, 4 , Emanuela Prado Ferraz 1
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Autologous cell‐based therapy for bone regeneration might be impaired by diabetes mellitus (DM) due to the negative effects on mesenchymal stem cells (MSCs) differentiation. Strategies to recover their osteogenic potential could optimize the results. We aimed to evaluate the effect of photobiomodulation (PBM) therapy on osteoblast differentiation of rats with induced DM. Bone marrow MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB, respectively). dOB were treated with PBM therapy every 72 hour (660 nm; 0.14 J; 20 mW; 0.714 W/cm2, and 5 J/cm2). Cell morphology, viability, gene and protein expression of osteoblastic markers, alkaline phosphatase (ALP) activity, and the mineralized matrix production of dOB‐PBM were compared to dOB. PBM therapy improved viability of dOB, increased the gene and protein expression of bone markers, the ALP activity and the mineralized matrix production. PBM therapy represents an innovative therapeutic approach to optimize the treatment of bone defects in diabetic patients.
中文翻译：
通过光生物调节疗法恢复糖尿病大鼠间充质干细胞的成骨分化潜能
由于对间充质干细胞（MSCs）分化产生负面影响，糖尿病（DM）可能会损害基于自体细胞的骨再生疗法。恢复其成骨潜能的策略可以优化结果。我们旨在评估光生物调节（PBM）治疗对诱导性DM大鼠成骨细胞分化的影响。分离健康和糖尿病大鼠的骨髓MSC，并将其分化为成骨细胞（分别为OB和dOB）。dOB每72小时用PBM治疗（660 nm; 0.14 J; 20 mW; 0.714 W / cm 2和5 J / cm 2）。将细胞形态，成骨细胞标志物的活力，基因和蛋白质表达，碱性磷酸酶（ALP）活性以及dOB-PBM的矿化基质产生与dOB进行了比较。PBM治疗改善了dOB的活力，增加了骨标志物的基因和蛋白质表达，ALP活性和矿化的基质生成。PBM治疗代表了一种创新的治疗方法，可以优化糖尿病患者骨缺损的治疗。
更新日期：2020-11-12
中文翻译：
通过光生物调节疗法恢复糖尿病大鼠间充质干细胞的成骨分化潜能
由于对间充质干细胞（MSCs）分化产生负面影响，糖尿病（DM）可能会损害基于自体细胞的骨再生疗法。恢复其成骨潜能的策略可以优化结果。我们旨在评估光生物调节（PBM）治疗对诱导性DM大鼠成骨细胞分化的影响。分离健康和糖尿病大鼠的骨髓MSC，并将其分化为成骨细胞（分别为OB和dOB）。dOB每72小时用PBM治疗（660 nm; 0.14 J; 20 mW; 0.714 W / cm 2和5 J / cm 2）。将细胞形态，成骨细胞标志物的活力，基因和蛋白质表达，碱性磷酸酶（ALP）活性以及dOB-PBM的矿化基质产生与dOB进行了比较。PBM治疗改善了dOB的活力，增加了骨标志物的基因和蛋白质表达，ALP活性和矿化的基质生成。PBM治疗代表了一种创新的治疗方法，可以优化糖尿病患者骨缺损的治疗。
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