Gelatin (Gel) is a protein polymer obtained from the hydrolysis of collagen. Gel was used to prepare a wound dressing material loaded with asiaticoside (AC), a trisaccharide triterpene from Centella asiatica (L.) Urban commonly used in wound healing and dermis reconstruction. To improve water solubility, AC was formed into an inclusion complexation with 2‐hydroxypropyl‐β‐cyclodextrin (HPβCD) in molar ratios of 1:0.5, 1:1, and 1:2. The AC/HPβCD complexes were then loaded into a standard Gel solution and glutaraldehyde was used as the crosslinking of the Gel matrix. Freeze‐drying was used to fabricate the mixed solutions into Gel scaffolds. Average pore sizes of the scaffolds ranged between 548 and 560 μm. The compressive modulus of the scaffolds increased with increasing HPβCD molar ratio. Swelling and weight loss of the scaffolds in water increased with longer submersion times. Highest cumulative released amount of AC was obtained from the scaffold incorporated with the AC/HPβCD complex at the molar ratio of 1:2. Indirect cytotoxicity assay with human dermal fibroblasts confirmed that AC/HPβCD complex‐loaded Gel scaffolds were non‐toxic to the cells. Results demonstrated the potential of AC/HPβCD complex‐loaded Gel scaffolds for use as wound dressing materials.

中文翻译：

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载有积雪草苷/ 2-羟丙基-β-环糊精复合物的明胶支架用作伤口敷料

明胶（Gel）是从胶原蛋白水解获得的蛋白质聚合物。凝胶用于制备载有积雪草苷（AC）（积雪草中的三糖三萜）的伤口敷料材料（L.）Urban通常用于伤口愈合和真皮重建。为了提高水溶性，AC与2-羟丙基-β-环糊精（HPβCD）形成摩尔比为1：0.5、1：1和1：2的包合物。然后将AC /HPβCD复合物加载到标准凝胶溶液中，并使用戊二醛作为凝胶基质的交联。冷冻干燥用于将混合溶液制成凝胶支架。支架的平均孔径在548至560μm之间。支架的压缩模量随HPβCD摩尔比的增加而增加。浸泡时间越长，支架在水中的溶胀和重量损失越大。从掺入AC /HPβCD复合物的支架以1∶2的摩尔比获得最高的AC累积释放量。用人类皮肤成纤维细胞进行的间接细胞毒性试验证实，AC /HPβCD复合物负载的凝胶支架对细胞无毒。结果表明，负载AC /HPβCD复合物的凝胶支架可用作伤口处理材料的潜力。