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A rapid, simple and ultrasensitive spectrofluorimetric method for the direct detection of metformin in real samples based on a nanoquenching approach
Luminescence ( IF 3.2 ) Pub Date : 2020-11-12 , DOI: 10.1002/bio.3982 Sina Azarian 1 , Masoomeh Shaghaghi 2 , Gholamreza Dehghan 1 , Nader Sheibani 3
Luminescence ( IF 3.2 ) Pub Date : 2020-11-12 , DOI: 10.1002/bio.3982 Sina Azarian 1 , Masoomeh Shaghaghi 2 , Gholamreza Dehghan 1 , Nader Sheibani 3
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Metformin (MET), as an oral antidiabetic and antihyperglycemic agent, is widely used to treat type II diabetes mellitus. Because of its increasing consumption, developing a fast, simple, and selective method to determine its concentration in biological samples (serum and urine) and pharmaceutical formulations (tablets) is of great interest. In this study, we used a FRET‐based fluorescent nanosensor (Tb–phen–AgNPs system) for sensitive detection of MET in tablet and serum samples. This method is based on the enhancing effect of MET on the emission intensity of the Tb–phen complex, which is quenched by AgNPs via energy transfer process (turn off–on mode). A good linear relationship between the MET concentration and enhanced emission intensity of the Tb–phen–AgNPs system was observed in the range of (0.75–3.7) × 10−6 M under optimum conditions. Limit of detection and limit of quantitation were calculated to be 0.43 × 10−6 M and 1.31 × 10−6 M, respectively. This method was successfully used to determine MET concentrations in pharmaceutical dosage form and in spiked serum sample. The obtained recoveries from pharmaceutical formulation and serum sample were in the range 86.75–98.97% and 85.10–100.96%, respectively. Collectively, our results indicated that the method described here is simple, sensitive, cost effective, and free from interference. Therefore, it can be used as an effective and routine method for the direct and rapid determination of MET levels in biological samples such as serum.
中文翻译：
基于纳米猝灭法的快速，简单和超灵敏的荧光光谱法直接检测实际样品中的二甲双胍
二甲双胍（MET）作为口服降糖和降糖药，被广泛用于治疗II型糖尿病。由于其消耗量的增加，开发一种快速，简单和选择性的方法来测定其在生物样品（血清和尿液）和药物制剂（片剂）中的浓度引起了人们的极大兴趣。在这项研究中，我们使用基于FRET的荧光纳米传感器（Tb–phen–AgNPs系统）对片剂和血清样品中的MET进行灵敏检测。该方法基于MET对Tb-phen配合物发射强度的增强作用，该配合物通过AgNP通过能量转移过程（关闭-开启模式）淬灭。在（0.75–3.7）×10 -6范围内，观察到MET浓度与Tb–phen–AgNPs系统增强的发射强度之间存在良好的线性关系。M在最佳条件下。检出限和定量限分别计算为0.43×10 -6 M和1.31×10 -6M。该方法已成功用于测定药物剂型和加标血清样品中的MET浓度。从药物制剂和血清样品中获得的回收率分别为86.75–98.97％和85.10–100.96％。总的来说，我们的结果表明，此处描述的方法简单，灵敏，具有成本效益并且没有干扰。因此，它可以用作直接快速测定生物样品（例如血清）中MET水平的有效常规方法。
更新日期：2020-11-12
中文翻译：
基于纳米猝灭法的快速，简单和超灵敏的荧光光谱法直接检测实际样品中的二甲双胍
二甲双胍（MET）作为口服降糖和降糖药，被广泛用于治疗II型糖尿病。由于其消耗量的增加，开发一种快速，简单和选择性的方法来测定其在生物样品（血清和尿液）和药物制剂（片剂）中的浓度引起了人们的极大兴趣。在这项研究中，我们使用基于FRET的荧光纳米传感器（Tb–phen–AgNPs系统）对片剂和血清样品中的MET进行灵敏检测。该方法基于MET对Tb-phen配合物发射强度的增强作用，该配合物通过AgNP通过能量转移过程（关闭-开启模式）淬灭。在（0.75–3.7）×10 -6范围内，观察到MET浓度与Tb–phen–AgNPs系统增强的发射强度之间存在良好的线性关系。M在最佳条件下。检出限和定量限分别计算为0.43×10 -6 M和1.31×10 -6M。该方法已成功用于测定药物剂型和加标血清样品中的MET浓度。从药物制剂和血清样品中获得的回收率分别为86.75–98.97％和85.10–100.96％。总的来说，我们的结果表明，此处描述的方法简单，灵敏，具有成本效益并且没有干扰。因此，它可以用作直接快速测定生物样品（例如血清）中MET水平的有效常规方法。
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