Phycocyanin isolated from Anabaena biomass was in‐vitro assayed for its antioxidant activity against DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and ABTS [2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid)] free radical, revealing maximum activities amounting to 77 and 80% at 1,000 µg/ml and SC₅₀ values about 96 and 111 µg/ml, respectively. A biological experiment was conducted, involving 40 male Wistar Albino rats, divided into five groups. Group I received only the basal diet as a normal control, while groups II, III, IV, and V were administrated intraperitoneal (IP) injection of a single dose of CCl₄ (50% in corn oil) at 0.5 ml/kg body weight. Subsequently, groups II, III, IV, and V received phycocyanin at 0.0, 25, 50, and 100 mg/kg body weight/day. CCl₄ induced considerable increases (p < .05) in the levels of serum ALT, AST, urea and creatinine, total lipid, and triglycerides coupled with significant reductions (p < .05) in serum antioxidant enzymes and some liver histopathological deformations compared to the negative control (group 1). Administration of Anabaena oryzae phycocyanin can counteract these CCl₄‐induced changes.

中文翻译：

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鱼腥藻藻蓝蛋白减轻四氯化碳诱导的大鼠肝细胞损伤和氧化应激

体外测定了从鱼腥藻生物质中分离出的藻蓝蛋白对DPPH（2,2-二苯基-1-吡啶甲基肼基）和ABTS [2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）]的抗氧化活性。自由基，显示最大活性在1,000 µg / ml时分别为77％和80％，SC ₅₀值分别约为96和​​111 µg / ml。进行了一项生物学实验，将40只雄性Wistar Albino大鼠分为5组。I组仅接受基础饮食作为正常对照组，而II，III，IV和V组则以0.5 ml / kg体重腹膜内（IP）注射单剂量的CCl ₄（玉米油中含量为50％） 。随后，第II，III，IV和V组接受0.0、25、50和100 mg / kg体重/天的藻蓝蛋白。氯仿_4个诱导的相当大的增加（P <  0.05）在血清ALT，AST，尿素，肌酐，总脂质的水平，并与显著减少（甘油三酯P < 血清抗氧化酶0.05）和一些肝组织病理学变形相比阴性对照（第1组）。施用 稻米藻 蓝蛋白可抵消这些CCl ₄诱导的变化。