Human Serum Albumin−Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA‐200c,Electroanalysis

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Human Serum Albumin−Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA‐200c
Electroanalysis ( IF 2.7 ) Pub Date : 2020-11-13 , DOI: 10.1002/elan.202060342
Oznur Akbal Vural ₁ , Yesim Tugce Yaman _{1,

2} , Gulcin Bolat ₂ , Serdar Abaci _{1,

2}

Affiliation

Here, human serum albumin conjugated gold nanoparticles (HSA−AuNPs) were synthesized by a simple route to develop an impedimetric sensor for miRNA‐200c detection based on a selective oligo‐hybridization process without any labeling. The synthetic DNA capture probe for miRNA‐200c was decorated onto the HSA−AuNPs modified pencil graphite electrodes. Impedimetric signals were monitored after the hybridization process between the DNA probe and target miRNA‐200c. HSA−AuNPs adsorption time, incubation time of the capture probe and hybridization time‐temperature were optimized. The proposed miRNA‐200c biosensor demonstrated proper sensitivity and selectivity, low detection limit (1.13 fM), good reproducibility and simple direct detection of miRNA‐200c in serum.

中文翻译：

基于人血清白蛋白-金纳米颗粒的阻抗传感器，用于miRNA-200c的灵敏检测

在这里，人血清白蛋白缀合的金纳米颗粒（HSA-AuNPs）通过一种简单的途径合成，以开发基于miRNA-200c检测的阻抗传感器，基于选择性的寡杂交过程而无需任何标记。用于miRNA-200c的合成DNA捕获探针被修饰在HSA-AuNPs修饰的铅笔石墨电极上。在DNA探针和靶标miRNA-200c之间的杂交过程之后，对阻抗信号进行监测。优化了HSA-AuNPs的吸附时间，捕获探针的孵育时间和杂交时间-温度。拟议的miRNA-200c生物传感器表现出适当的灵敏度和选择性，低检测限（1.13 fM），良好的重现性和简单的直接检测血清中的miRNA-200c的能力。

更新日期：2020-11-13

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