Cover Feature: Self‐Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid‐Liquid Phase Separation (ChemBioChem 23/2020),ChemBioChem

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Cover Feature: Self‐Emergent Protocells Generated in an Aqueous Solution with Binary Macromolecules through Liquid‐Liquid Phase Separation (ChemBioChem 23/2020)
ChemBioChem ( IF 2.6 ) Pub Date : 2020-11-13 , DOI: 10.1002/cbic.202000760
Hiroki Sakuta ₁ , Fumika Fujita ₁ , Tsutomu Hamada ₂ , Masahito Hayashi ₃ , Kingo Takiguchi ₄ , Kanta Tsumoto ₅ , Kenichi Yoshikawa _{1,

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Affiliation

A cell‐like structure or protocell emerges spontaneously by simply mixing DNAs, phospholipids and two kinds of hydrophilic macromolecules: PEG and dextran (DEX). The top middle picture shows a water/water microdroplet (the DEX‐rich phase surrounded by the PEG‐rich phase) generated through the depletion effect of the different macromolecules. By the addition of DNAs to the solution, the DEX‐rich droplet autonomously takes DNAs inside (see the droplet bottom right). In the coexistence of phospholipids, they cover the droplets, and a stable phospholipid vesicle entrapping DNAs appears in a self‐organized manner (see the protocell bottom left). More information can be found in the communication by K. Takiguchi, K. Tsumoto, et al.

中文翻译：

水溶液与二元大分子通过液相分离产生的自生原始细胞

只需将DNA，磷脂和两种亲水性大分子：PEG和右旋糖酐（DEX）混合，即可自发出现细胞样结构或原生细胞。顶部中间的图片显示了水/水微滴（DEX富集相被PEG富集相包围）通过不同大分子的耗尽效应而产生。通过向溶液中添加DNA，富含DEX的液滴可以自动将DNA吸收到内部（请参见液滴右下角）。在磷脂共存的情况下，它们覆盖了液滴，并且以自组织的方式出现了稳定的包裹磷脂囊泡的DNA（请参见左下方的原始细胞）。可以在K. Takiguchi，K。Tsumoto等人的来文中找到更多信息。

更新日期：2020-12-01

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