The objective of the study was to isolate and identify bacteria having plant growth promoting rhizobacteria (PGPR) characteristics from the rhizosphere of wild orchids. Orchid plants belonging to the species of Anacamptis pyramidalis, Himantoglossum caprinum, Limodorum abortivum, Platanthera bifolia, Serapias vomeracea subsp. laxiflora, Spiranthes spiralis, Ophrys apifera, Ophrys sphegodes, Orchis coriophora, Orchis laxiflora, Orchis provincialis, Orchis tridentata from the Black Sea region (Samsun, Turkey). Rhizobacteria were isolated from the rhizosphere of orchids and assayed for phosphate solubilization, 1 aminocyclopropane-1-carboxylate (ACC) deaminase activity and indole-3-acetic acid (IAA) production. Out of 111 rhizobacteria, 65 solubilized phosphate, 32 had ACC deaminase activity, 83 produced IAA. Sixteen of the isolates showed all the traits. Comparing sequences of 16S rDNA against the database with BLAST, the most common genera were Bacillus, Paenibacillus, Pseudomonas, Acinetobacter, Agrobacterium and Stenotrophomonas. This is the first study to comprehensively evaluate the PGPR properties of strains isolated from wild orchid rhizosphere.

该研究的目的是从野生兰花的根际中分离和鉴定具有植物生长促进根际细菌（PGPR）特性的细菌。属于Anacamptis pyramidalis，Himantoglossum caprinum，Limodorum abortivum，Platanthera bifolia，Serapias vomeracea亚种的兰花植物。laxiflora，Spiranthes spiralis，Ophrys apifera，Ophrys sphegodes，Orchis coriophora，Orchis laxiflora，Orchis provinceis，Orchis tridentata来自黑海地区（土耳其萨姆松）。从兰花的根际中分离出根际细菌，并分析其磷酸盐增溶作用，1个氨基环丙烷-1-羧酸盐（ACC）脱氨酶活性和吲哚-3-乙酸（IAA）的产生。在111种根瘤菌中，有65种溶解的磷酸盐，有32种具有ACC脱氨酶活性，有83种产生了IAA。分离株中有16个表现出所有特征。针对与BLAST比较数据库中的16S rDNA序列，最常见的属有芽孢杆菌，芽孢杆菌，假单胞菌，不动杆菌属，根和嗜。这是首次全面评估从野生兰花根际分离菌株的PGPR特性的研究。