Since attributed to the involvement in important physiological and pathological processes, hydrogen sulfide (H₂S) is regarded as another gasotransmitter like nitric oxide (NO) and carbon monoxide (CO). Traditional H₂S detection methods are limited by the fast volatility and catabolism of H₂S and difficult to real-time detect H₂S in biological systems. Hence, numerous small-molecule fluorescent probes have been developed. Besides high sensitivity, the selectivity, response capability and optical performance are improved by combining new response groups and extending the emission wavelength to near infrared (NIR) region. Moreover, probes tend to selectively locate in a specific organelle, which detect H₂S more accurately and contribute to understand the physiological roles of H₂S in diseases. In this review, recent developments of small-molecule fluorescent probes for H₂S imaging were summarized, focusing on researches conducted between January 2018 and June 2020, and their structures and biological applications were highlighted.

由于归因于重要的生理和病理过程，硫化氢（H ₂ S）被认为是另一种气体传输剂，如一氧化氮（NO）和一氧化碳（CO）。传统ħ ₂小号检测方法是通过H的快速波动和分解代谢局限于₂ S和难以实时检测ħ ₂ S IN的生物系统。因此，已经开发了许多小分子荧光探针。除了高灵敏度外，还通过组合新的响应组并将发射波长扩展到近红外（NIR）区域来提高选择性，响应能力和光学性能。而且，探针倾向于选择性地定位在检测H ₂的特定细胞器中。S更准确，有助于理解H ₂ S在疾病中的生理作用。在这篇综述中，总结了用于H ₂ S成像的小分子荧光探针的最新发展，重点是2018年1月至2020年6月之间进行的研究，并着重介绍了其结构和生物学应用。