MST is an orally administered selective tyrosine kinase inhibitor. It has emerged as a promising drug for multiple diseases including cancer and inflammation in either human or veterinary medicine. Five new and simple UV spectrophotometric methods were developed for its determination in bulk and in pharmaceutical tablets. These methods are based on measuring the absorbance of masitinib in either zero order or first, second, third or fourth derivative spectra. Measurements are optimized so as to minimize excipients' interferences. The methods are suitable for micro-analysis of masitinib. The proposed methods were validated according to the ICH-Q2(R1) guidelines and was successfully applied for determination of masitinib in laboratory prepared tablet. The presented methods are simple, fast, cost‐effective and suitable for routine pharmaceutical analysis. Moreover, two derivative spectrophotometric-based methods were developed for identification of masitinib, the derivative ratio method and log-A derivative method. The impact of the developed methods on the environment was assessed by both analytical Eco-Sale and the Green Analytical Procedure Index (GAPI). The present work proves how derivative spectrophotometry could greatly extract qualitative and quantitative information from UV spectra.

MST是口服的选择性酪氨酸激酶抑制剂。它已成为人类或兽医学中针对多种疾病（包括癌症和炎症）的有前途的药物。开发了五种新的简单的紫外分光光度法，用于批量测定和片剂测定。这些方法基于以零阶或一阶，二阶，三阶或四阶导数光谱测量马赛替尼的吸光度。优化了测量，以最大程度地减少辅料的干扰。该方法适用于马赛替尼的微分析。根据ICH-Q2（R1）指南对提出的方法进行了验证，并成功地用于实验室制备的片剂中马赛替尼的测定。提出的方法简单，快速，具有成本效益，适用于常规药物分析。此外，还开发了两种基于分光光度法的马赛替尼鉴定方法，即导数比法和log-A导数法。通过分析性生态销售和绿色分析程序指数（GAPI）评估了开发方法对环境的影响。本工作证明了导数分光光度法如何从紫外光谱中极大地提取定性和定量信息。