Nowadays, ozone has been widely applied in industry and medical therapies. However, excessive exposure to ozone can lead to lung dysfunction and many respiratory symptoms. As a member of reactive oxygen species (ROS), ozone also was involved in various physiology and pathology process. Given the fact of this, the effective detection of ozone in the atmosphere and biological system is of vital significance. Herein, we reported a novel dicyanomethylene-4H-pyran (DCM)-based fluorescent probe DCM-O₃ with butenyl being the recognition moiety for monitoring ozone. The probe displayed high selectivity towards ozone, and its response towards ozone could be completed within 5 min under the optimal condition. Besides, a good linear correlation was obtained between the ozone concentrations (0 to 50 μM) and the corresponding fluorescent intensity at 560 nm, and the limit of detection (LOD) was calculated to be 6.2 ×10^-7 M. Moreover, the probe DCM-O₃ showed low cytotoxicity and was successfully applied to detect ozone in live cells. Given all the merits, the probe DCM-O₃ could function as a robust tool for researchers to investigate ozone-related diseases in the complex biological environment.

如今，臭氧已广泛应用于工业和医学治疗中。但是，过量暴露于臭氧会导致肺功能障碍和许多呼吸道症状。作为活性氧（ROS）的成员，臭氧还参与了各种生理和病理过程。鉴于这一事实，有效检测大气和生物系统中的臭氧至关重要。在这里，我们报道了一种新型的基于二氰基亚甲基-4H-吡喃（DCM）的荧光探针DCM-O ₃其中丁烯基是监测臭氧的识别部分。该探针显示出对臭氧的高度选择性，并且在最佳条件下，其对臭氧的响应可以在5分钟内完成。此外，臭氧浓度（0至50μM）与相应的560 nm荧光强度之间具有良好的线性相关性，计算的检出限（LOD）为6.2×10 ^-7M。 DCM-O ₃具有低细胞毒性，已成功应用于检测活细胞中的臭氧。考虑到所有优点，DCM-O ₃探针可作为研究人员研究复杂生物环境中与臭氧有关的疾病的强大工具。