This study aims to analyze the combined form, detoxification, and adsorption mechanism of Pb in Cladophora rupestris subcells. The chemical form analysis at different concentrations (0, 0.5, 1.0, 2.5, 5.0, 7.5, and 10 mg/L) indicated that most of the Pb (37%–76%) were integrated with oxalate and undissolved phosphate, which were important to the detoxification of C. rupestris. The characterization of Pb (0, 0.5, and 5.0 mg/L) at the subcellular was conducted via Fourier-transform infrared spectroscopy (FTIR), Three-dimensional excitation-emission matrix spectroscopy (3D-EEM), and protein secondary structure fitting. Results revealed that Pb-polysaccharides ((C₆H₅)-OO-Pb-OH, C-O-Pb, and symmetric Pb-O-Pb), Pb-functional-groups ((C₆H₅)-COO-Pb and (C₆H₅)-P=O-Pb), and Pb-protein complexes (OH-C₇H₆-CN-Pb-COOH, C₉H₁₀-NH-CN-C=O-Pb, Pb-S-C, and Pb-S) were formed. The cell wall produced transport proteins, such as metallothionein and glutathione, which bound and helped Pb²⁺ enter the cell. After entering the soluble fraction, the Pb–organic acid ((C₆H₅)-COO-Pb, (C₆H₅)-O-Pb, and (C₆H₅)-P=O-Pb) and Pb-sulfhydryl compound (Pb-S-C/Pb-S) assumed the most important role in resisting the toxicity of Pb²⁺. Pb²⁺ was absorbed in the organelle and formed (C₆H₅)-C-O-Pb and (C₆H₅)-P=O-Pb, and complexed with protein (Pb-C-N) when treated with 5.0 mg/L Pb. Results could help understand the role of subcellular fraction in the algal adaptation to stressful heavy metal conditions.

本研究旨在分析红果刺柏亚细胞中铅的结合形式，解毒作用和吸附机理。在不同浓度（0、0.5、1.0、2.5、5.0、7.5和10 mg / L）下的化学形态分析表明，大多数Pb（37％–76％）与草酸盐和未溶解的磷酸盐结合在一起，这很重要对卢氏梭菌排毒。通过傅立叶变换红外光谱（FTIR），三维激发发射矩阵光谱（3D-EEM）和蛋白质二级结构拟合，对亚细胞中的Pb（0、0.5和5.0 mg / L）进行了表征。结果显示Pb多糖（（C ₆ H ₅）-OO-Pb-OH，CO-Pb和对称Pb-O-Pb），Pb官能团（（C₆ H ₅）-COO-Pb和（C ₆ H ₅）-P = O-Pb）和Pb蛋白复合物（OH-C ₇ H ₆ -CN-Pb-COOH，C ₉ H ₁₀ -NH-CN形成-C ＝ O-Pb，Pb-SC和Pb-S。细胞壁产生转运蛋白，例如金属硫蛋白和谷胱甘肽，它们结合并帮助Pb ²⁺进入细胞。进入可溶性级分后，Pb-有机酸（（C ₆ H ₅）-COO-Pb，（C ₆ H ₅）-O-Pb和（C ₆ H ₅）-P = O-Pb）和Pb -巯基化合物（Pb-SC / Pb-S）在抵抗Pb毒性中起着最重要的作用²⁺。Pb ²⁺被细胞器吸收并形成（C ₆ H ₅）-CO-Pb和（C ₆ H ₅）-P = O-Pb，并在用5.0 mg / L处理时与蛋白质（Pb-CN）络合铅 结果可能有助于了解亚细胞部分在藻类适应重金属环境中的作用。