Herein, a novel and rapid fluorometric nanoprobe was constructed for quantitation of dopamine (DA) in presence of biologically interfering compounds. The nanoprobe based on synthesis of yellow emissive nitrogen doped graphene quantum dots (N@GQDs) by advanced thermal driven oxidation. After that, the synthesized N@GQDs was capped with β-cyclodextrin (β-CD), followed by interaction with pyridoxal (PYL) vitamin B₆ cofactor. This interaction resulted in diminishing the yellow fluorescence of β-CD/N@GQDs, and appearance of blue emission peak at 420 nm. Upon addition of DA, the blue emission of β-CD/N@GQDs was increased after excitation at λ = 330 nm. Under optimum conditions, the nanoprobe exhibited a linear range of 0.36–400 nM with limit of detection (LOD) of 0.117 nM. In addition, the fluorescent nanoprobe shows high selectivity and can be used for detection of DA in complicated biological matrices and human serum. This strategy might provide a potential tool for clinical diagnosis and biomedical research for DA related diseases.

本文中，构建了一种新型的快速荧光纳米探针，用于在存在生物干扰化合物的情况下定量多巴胺（DA）。该纳米探针基于通过先进的热驱动氧化法合成黄色发射氮掺杂的石墨烯量子点（N @ GQDs）。之后，用β-环糊精（β-CD）将合成的N @ GQDs封端，然后与吡ido醛（PYL）维生素B ₆相互作用辅助因子。这种相互作用导致β-CD/ N @ GQDs的黄色荧光减弱，并在420 nm处出现蓝色发射峰。加入DA后，在λ= 330 nm激发后，β-CD/ N @ GQDs的蓝色发射增加。在最佳条件下，纳米探针的线性范围为0.36–400 nM，检出限（LOD）为0.117 nM。此外，荧光纳米探针显示出高选择性，可用于检测复杂的生物基质和人血清中的DA。该策略可能为DA相关疾病的临床诊断和生物医学研究提供潜在的工具。