The aim of the present study was to determine the effects of serum total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) on counts of corpus luteum, transferable embryo, and embryo quality in cows treated with superovulation protocol. A total of 60 Simmental cows (postpartum 90 to 120 days) were used. The donors were treated with a 9 day-progesterone based estrus synchronization protocol. On the 7th day of the progesterone treatment, donors were treated with FSH twice daily as decreasing doses for 4 days. On day 9, PGF_2α injection was performed in the morning and progesterone source was removed in the evening. The cows were inseminated twice artificially on day 11 at 12 hours interval. On the day of artificial insemination, blood samples were collected to determine serum TAS, TOS and OSI concentrations. Uterine flushing was performed on day 7 after artificial insemination. Serum TAS, TOS and OSI concentrations of cows were 1.62 μmol/L, 5.44 μmol/L and 0.45 AU, respectively. There was a positive correlation between TAS concentration and the counts of transferable embryo, Code I and II quality embryo (P<0.05). TOS and OSI concentrations were negatively correlated with the counts of transferable embryo and Code I embryo, and positively correlated with unfertilized oocyte counts (P<0.05). As a result, it was concluded that TAS, TOS, and OSI affected the counts of transferable embryo, embryo quality, and unfertilized oocyte in donor cows.

本研究的目的是确定用SDS处理的母牛的血清总抗氧化剂状态（TAS），总氧化剂状态（TOS）和氧化应激指数（OSI）对黄体数量，可转移胚胎和胚胎质量的影响。超排卵协议。总共使用了60只西门塔尔牛（产后90到120天）。用基于9天孕酮的发情同步方案治疗供体。在孕酮治疗的第7天，每天两次以递减剂量FSH治疗供体，持续4天。在第9天，_PGF2α早上进行注射，晚上去除孕激素来源。在第11天以12小时的间隔对母牛进行两次人工授精。在人工授精的当天，收集血液样本以确定血清TAS，TOS和OSI浓度。人工授精后第7天进行子宫冲洗。奶牛的血清TAS，TOS和OSI浓度分别为1.62μmol/ L，5.44μmol/ L和0.45 AU。TAS浓度与可移植胚胎，Code I和II质量胚胎的数量呈正相关（P <0.05）。TOS和OSI浓度与可移植胚胎和Code I胚胎的数量呈负相关，与未受精卵母细胞的数量呈正相关（P<0.05）。结果得出结论，TAS，TOS和OSI影响了供体母牛的可移植胚胎数，胚胎质量和未受精卵母细胞。