Protein sequence comparison of human and non-human primate tooth proteomes,Journal of Proteomics

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Protein sequence comparison of human and non-human primate tooth proteomes
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-11-13 , DOI: 10.1016/j.jprot.2020.104045
Carine Froment ₁ , Clément Zanolli ₂ , Mathilde Hourset ₃ , Emmanuelle Mouton-Barbosa ₁ , Andreia Moreira ₄ , Odile Burlet-Schiltz ₁ , Catherine Mollereau ₄

Affiliation

In the context of human evolution, the study of proteins may overcome the limitation of the high degradation of ancient DNA over time to provide biomolecular information useful for the phylogenetic reconstruction of hominid taxa. In this study, we used a shotgun proteomics approach to compare the tooth proteomes of extant human and non-human primates (gorilla, chimpanzee, orangutan and baboon) in order to search for a panel of peptides able to discriminate between taxa and further help reconstructing the evolutionary relationships of fossil primates. Among the 25 proteins shared by the five genera datasets, we found a combination of peptides with sequence variations allowing to differentiate the hominid taxa in the proteins AHSG, AMBN, APOA1, BGN, C9, COL11A2, COL22A1, COL3A1, DSPP, F2, LUM, OMD, PCOLCE and SERPINA1. The phylogenetic tree confirms the placement of the samples in the appropriate genus branches. Altogether, the results provide experimental evidence that a shotgun proteomics approach on dental tissue has the potential to detect taxonomic variation, which is promising for future investigations of uncharacterized and/or fossil hominid/hominin specimens.

Significance

A shotgun proteomics approach on human and non-human primate teeth allowed to identify peptides with taxonomic interest, highlighting the potential for future studies on hominid fossils.

中文翻译：

人类和非人类灵长类动物牙齿蛋白质组的蛋白质序列比较

在人类进化的背景下，蛋白质的研究可以克服古代DNA随时间的高度降解的局限性，从而提供可用于人类分类系统重建的生物分子信息。在这项研究中，我们使用a弹枪蛋白质组学方法比较了现存的人类和非人类灵长类动物（大猩猩，黑猩猩，猩猩和狒狒）的牙齿蛋白质组，以寻找能够区分类群并进一步帮助重建的肽组。化石灵长类动物的进化关系。在五个属数据集共享的25种蛋白质中，我们发现了具有序列变异的多肽组合，从而可以区分蛋白质AHSG，AMBN，APOA1，BGN，C9，COL11A2，COL22A1，COL3A1，DSPP，F2，LUM ，OMD，PCOLCE和SERPINA1。系统发育树确认了样品在适当属分支中的位置。总而言之，这些结果提供了实验证据，表明在牙齿组织上使用shot弹枪蛋白质组学方法具有检测生物分类学变异的潜力，这有望在将来对未鉴定和/或化石原始人种/人种素样本进行研究。