Abstract This review summarizes the recent advances in the enantioselective separation of small molecules using biomolecules/macromolecules built up by natural inherent chiral moieties. Although different kinds of chiral selectors are known to date, the microspheres based on biomolecules have received paramount importance in view of their green synthesis, selective and sensitive separation of enantiomers from the racemic mixture. Direct separation of enantiomers devoid of using high-performance liquid chromatography adds to the elegance of this approach. We have covered the preparation of various biomolecules spheres by different methods. The enantiomeric separation of amino acids, drugs and other racemates and the underlying plausible mechanism is discussed.

中文翻译：

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用于对映分离应用的生物分子/大分子微球

摘要 本综述总结了利用天然手性部分构建的生物分子/大分子对小分子进行对映选择性分离的最新进展。尽管迄今为止已知不同种类的手性选择剂，但鉴于其绿色合成、对映异构体与外消旋混合物的选择性和灵敏分离，基于生物分子的微球已获得了极为重要的地位。无需使用高效液相色谱直接分离对映异构体增加了这种方法的优雅。我们已经介绍了通过不同方法制备各种生物分子球体。讨论了氨基酸、药物和其他外消旋体的对映体分离以及潜在的可能机制。