To examine the applicability of fluorinated membrane-forming phospholipids to reconstitution matrices for functional membrane proteins, the membrane properties of a synthetic ether-type phosphatidylcholine (PC) bearing partially fluorinated C₁₈-monoacetylenic (octadec-9-ynyl) chains, DF₈CCH₈PC, were compared with those of its non-fluorinated counterpart, DH₈CCH₈PC. Light-harvesting 2 complex (LH2) and the light-harvesting 1‒reaction center core complex (LH1-RC) isolated from purple photosynthetic bacteria were employed as probe membrane proteins to evaluate the extent to which their reconstitution into DF₈CCH₈PC membranes could proceed. DF₈CCH₈PC formed more expanded and more stable fluid monolayers than DH₈CCH₈PC at the air-water interface at 25 °C; the former PC molecule occupied an area of ca. 0.70 nm² at a collapse pressure, π_c, of 52 mN/m, while the latter occupied an area of ca. 0.55 nm² at a π_c of 45 mN/m. In contrast, the molecular motion detected using fluorescent probes was much more restricted in DF₈CCH₈PC bilayers than in DH₈CCH₈PC ones. Although the reconstitution efficiencies of both LH2 and LH1-RC into DF₈CCH₈PC bilayers were lower than those into DH₈CCH₈PC bilayers, the membrane proteins incorporated into DF₈CCH₈PC bilayers showed increased thermostability. The increased thermostability of these proteins in fluorinated PC membranes might be due to the restricted molecular motion in the hydrophobic chains. The results of this study suggest that partially fluorinated PCs can be useful materials for the construction of lipid‒functional membrane protein assemblies including large membrane protein complexes, such as LH1-RC, for biotechnological applications.

为了检查氟化的成膜磷脂对于功能性膜蛋白的重构基质的适用性，带有部分氟化的C _18-单乙炔（_十八烷基-9-炔基）链，DF ₈ CCH的合成醚型磷脂酰胆碱（PC）的膜性能_将8 PC与非氟化产品DH ₈ CCH ₈ PC进行了比较。从紫色光合细菌中分离出的光捕获2配合物（LH2）和光捕获1反应中心核心复合物（LH1-RC）被用作探针膜蛋白，以评估其重组为DF ₈ CCH ₈ PC膜的程度可以继续。DF ₈CCH ₈ PC形成更多的膨胀和更稳定的流体单层比DH ₈ CCH ₈在25℃下在空气-水界面的PC; 前者PC分子占据的面积CA。0.70纳米²在坍塌压力，π _Ç，52 mN / m的，而后者所占据的区域CA。0.55纳米²以π _Ç 45 mN / m的。相反，在DF ₈ CCH ₈ PC双层中，使用荧光探针检测到的分子运动比在DH ₈ CCH _8中受到的限制更大。PC的。尽管LH2和LH1-RC进入DF ₈ CCH ₈ PC双层的重建效率均低于DH ₈ CCH ₈ PC双层，但掺入DF ₈ CCH ₈ PC双层的膜蛋白显示出更高的热稳定性。这些蛋白质在氟化PC膜中提高的热稳定性可能是由于疏水链中的分子运动受到限制。这项研究的结果表明，部分氟化的PCs可以用于构建脂质功能性膜蛋白组件，包括用于生物技术的大型膜蛋白复合物（例如LH1-RC）。