Abstract The production of sugarcane, a globally important crop, is likely to be impacted by changes in climate including higher average temperatures and decreased rainfall in regions such as the northeast and southeast of Brazil. This metatranscriptome study investigated the effect of water stress on the microbiota associated with the sugarcane rhizosphere, in order to obtain new information about plant-microbiota interactions and the ways that microorganisms could help plants to survive under adverse conditions. The results indicated that water stress increased the exudation of organic acids from the plant roots and changed the microbial gene expression in the rhizosphere. Decreased expression of genes related to growth and energy metabolism was mainly evident in samples submitted to eight days of water stress and was classified to the genera Sorangium and Rhodanobacter. Genes associated with functions such as flagella mobility, cell adhesion, and protein degradation showed increased expression in the same time period, with these transcripts classified to the genus Aequorivita. This genus also showed a positive correlation with the organic acids exuded by the plant. These results contribute to an understanding of the dynamics of the rhizosphere microbial community under normal and water stress conditions, indicating possible microbial groups that may play important roles in the maintenance of sugarcane under water stress.

中文翻译：

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甘蔗根际微生物群对不同水平水分胁迫的响应

摘要 甘蔗是一种全球重要的作物，其生产可能会受到气候变化的影响，包括巴西东北部和东南部等地区的平均气温升高和降雨量减少。这项宏转录组研究调查了水分胁迫对甘蔗根际相关微生物群的影响，以获得关于植物-微生物群相互作用的新信息，以及微生物帮助植物在不利条件下生存的方式。结果表明，水分胁迫增加了植物根系有机酸的渗出，改变了根际微生物基因的表达。与生长和能量代谢相关的基因表达减少主要在经受 8 天水分胁迫的样品中表现出来，并被归类为 Sorangium 和 Rhodanobacter 属。与鞭毛运动、细胞粘附和蛋白质降解等功能相关的基因在同一时期内表达增加，这些转录本归类为水母藻属。该属还与植物分泌的有机酸呈正相关。这些结果有助于了解正常和水分胁迫条件下根际微生物群落的动态，表明可能在水分胁迫下甘蔗维持中发挥重要作用的微生物群。细胞粘附和蛋白质降解在同一时间段内表现出增加的表达，这些转录本归类为水母藻属。该属还与植物分泌的有机酸呈正相关。这些结果有助于了解正常和水分胁迫条件下根际微生物群落的动态，表明可能在水分胁迫下甘蔗维持中发挥重要作用的微生物群。细胞粘附和蛋白质降解在同一时间段内表现出增加的表达，这些转录本归类为水母藻属。该属还与植物分泌的有机酸呈正相关。这些结果有助于了解正常和水分胁迫条件下根际微生物群落的动态，表明可能在水分胁迫下甘蔗维持中发挥重要作用的微生物群。