Abstract Clay nanocomposite hydrogels show great potential in tissue engineering applications due to their large layer space, cationic exchange capability, antimicrobial activity and advances in cell attachment and cell proliferation. Bentonite is a natural clay composed of a multi-layered structure with calcium and sodium cations between the tetrahedral and the octahedral layers. This article presents the development of a nanocomposite hydrogel from bentonite nanoparticles and gelatin methacryloyl (GelMA) for tissue engineering applications by investigating its mechanical, morphological and biocompatibility properties. In this research, two chemical treatment methods were studied on bentonite to manipulate it's drying density and its moisture content as an aim to enhance the mechanical stiffness of bentonite. Compression test was used to compare the mechanical properties of calcium and sodium bentonite-GelMA. It is clear that sodium bentonite enhances the mechanical properties of pure GelMA two times, while calcium bentonite showed no effect. The microstructure analysis showed a difference in the pore size when the concentrations of bentonite were varied. The 3D cell encapsulation experiment showed a maintenance of high cell viability over 5 days. The content covered in this paper will be an introduction for bentonite-GelMA nanocomposite hydrogels for further research studies in tissue engineering.

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用于组织工程的膨润土-明胶纳米复合混合水凝胶的开发

摘要 粘土纳米复合水凝胶由于其层空间大、阳离子交换能力、抗菌活性以及在细胞附着和细胞增殖方面的进步，在组织工程应用中显示出巨大的潜力。膨润土是一种天然粘土，由四面体和八面体层之间具有钙和钠阳离子的多层结构组成。本文通过研究其机械、形态和生物相容性特性，介绍了用于组织工程应用的膨润土纳米颗粒和明胶甲基丙烯酰 (GelMA) 纳米复合水凝胶的开发。在这项研究中，研究了两种化学处理方法对膨润土进行处理，以控制其干燥密度和含水量，以提高膨润土的机械刚度。压缩试验用于比较钙钠膨润土-GelMA 的力学性能。很明显，钠基膨润土使纯 GelMA 的机械性能提高了两倍，而钙基膨润土则没有影响。微观结构分析表明，当膨润土的浓度不同时，孔径存在差异。3D 细胞封装实验表明，在 5 天内保持了高细胞活力。本文涵盖的内容将介绍膨润土-GelMA 纳米复合水凝胶，用于组织工程的进一步研究。微观结构分析表明，当膨润土的浓度不同时，孔径存在差异。3D 细胞封装实验表明，在 5 天内保持了高细胞活力。本文涵盖的内容将介绍膨润土-GelMA 纳米复合水凝胶，用于组织工程的进一步研究。微观结构分析表明，当膨润土的浓度不同时，孔径存在差异。3D 细胞封装实验表明，在 5 天内保持了高细胞活力。本文涵盖的内容将介绍膨润土-GelMA 纳米复合水凝胶，用于组织工程的进一步研究。