Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 µM linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H₂O₂ treatment groups was detected in 4h 600µM H₂O₂ in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.

脂肪细胞死亡在肥胖症发展中很重要。了解和预防脂肪细胞死亡可能是治疗中的分子方法。在这项研究中，我们旨在了解Niban基因在脂肪细胞中的作用，该基因作为一种抗凋亡分子，可应对压力条件。用不同剂量的亚油酸，过氧化氢和乙醇处理3T3-L1脂肪细胞；实时监测iCELLingence系统检查细胞的增殖和增殖情况。通过q-PCR测量基因表达水平。作为对24小时480 µM亚油酸处理的响应，发现Niban基因表达高于对照组（p = 0.008），而测定的24小时90 mM乙醇处理低于对照组（p = 0.008）。H ₂ O中Niban基因表达的最高值与对照组相比，在4h600μMH ₂ O ₂中检测到_2个治疗组（p = 0.008）。为了了解Niban在脂肪形成中的作用，比较了前脂肪细胞和晚期脂肪积累脂肪细胞之间的Niban基因表达，并确定它们之间存在显着差异（p = 0.042）。我们的结果表明，Niban可能参与了脂肪细胞的应激反应过程。但是，Niban的确切分子作用需要进一步研究。