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Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2020-11-13 , DOI: 10.1007/s11033-020-05992-5
Mehtap Cevik 1 , Meliha Koldemir Gunduz 2 , Gokce Deliorman 3 , Belgin Susleyici 1
Affiliation  

Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 µM linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600µM H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.



中文翻译:

尼班基因表达的变化作为对3T3-L1脂肪细胞中应激条件的响应

脂肪细胞死亡在肥胖症发展中很重要。了解和预防脂肪细胞死亡可能是治疗中的分子方法。在这项研究中,我们旨在了解Niban基因在脂肪细胞中的作用,该基因作为一种抗凋亡分子,可应对压力条件。用不同剂量的亚油酸,过氧化氢和乙醇处理3T3-L1脂肪细胞;实时监测iCELLingence系统检查细胞的增殖和增殖情况。通过q-PCR测量基因表达水平。作为对24小时480 µM亚油酸处理的响应,发现Niban基因表达高于对照组(p = 0.008),而测定的24小时90 mM乙醇处理低于对照组(p = 0.008)。H 2 O中Niban基因表达的最高值与对照组相比,在4h600μMH 2 O 2中检测到2个治疗组(p = 0.008)。为了了解Niban在脂肪形成中的作用,比较了前脂肪细胞和晚期脂肪积累脂肪细胞之间的Niban基因表达,并确定它们之间存在显着差异(p = 0.042)。我们的结果表明,Niban可能参与了脂肪细胞的应激反应过程。但是,Niban的确切分子作用需要进一步研究。

更新日期:2020-11-13
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