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Suppression of chili leaf curl virus (ChLCV) incidence in chili ( Capsicum annuum L.) across Bangladesh via manipulated planting date and spacing
Journal of Plant Diseases and Protection ( IF 2 ) Pub Date : 2020-11-13 , DOI: 10.1007/s41348-020-00397-9
Shimul Das , Mahfuzur Rahman , Prosanta Kumar Dash , Md. Mostofa Kamal

A 2-year factorial experiment was undertaken in RARS, Jessore in 2019 and 2020 following randomized complete block design with three replicates to evaluate the impact of planting date and spacing on ChLCV disease development and its vector whitefly abundance in chili (Capsicum annuum) modulating chili growth and yield attributes. The field experiments were found consistent between the years improving the reliability and generalizability of the data. The factors planting date and spacing separately produced statistical variation in ChLCV epidemiological parameters along with growth and yield attributing characters. Of the three chili planting dates, early planting (January 1) significantly suppressed ChLCD incidence, severity and its vector whitefly abundance over mid (February 1) and late planting (March 1) dates. Among four spacings, spacious plant spacing (50 × 60 cm) performed significantly better than other spacings in reducing ChLCD incidence and thereby expediting chili growth and yield. No statistical variation was documented between two narrow spacings of 50 × 30 cm and 50 × 40 cm in most of the studied parameters. Regression analyses depicted AUSiPC and AUDPC declined by 13.07 and 12.66 with each unit increase in plant spacing ensuring good fits of the models. A three-variables contour plot illustrated how vector whitefly abundance in chili stimulated ChLCD incidence and reduced chili yield while a 3-D wireframe pointed out that ChLCD incidence accelerated both AUSiPC and AUDPC. PCA analysis identified the major components of the studied parameters impacting disease incidence and chili yield. Cluster analyses well supported the PCA analysis and distinctly branched all the studied parameters into two, one associated with chili yield promoting characters and another with yield constraining ChLCD epidemiological attributes positioned in different nodes with variable similarity matrix. Molecular PCR analysis confirmed the presence of ssDNA (+) ChLCV in sampled plants.



中文翻译:

通过操纵播种日期和间隔,抑制孟加拉国辣椒(Capsicum annuum L.)中辣椒叶片卷曲病毒(ChLCV)的发生

2年的阶乘实验进行中RARS,杰索尔在2019年和2020年以下完全随机区组设计,3次重复,以评估播种期的影响和间距上ChLCV病情发展及其载体粉虱丰辣椒(甜椒)调节辣椒的生长和产量属性。多年的实地实验发现,在提高数据的可靠性和通用性的年份之间是一致的。种植日期和间距的因素分别在ChLCV流行病学参数以及生长和产量归因特征上产生统计差异。在这三个辣椒的播种日期中,早期播种(1月1日)在播种中期(2月1日)和播种后期(3月1日)显着抑制了ChLCD的发生率,严重性及其媒介粉虱的丰度。在四个间距中,宽敞的植物间距(50×60厘米)在降低ChLCD发生率,从而加快辣椒生长和产量方面表现出明显优于其他间距。在大多数研究参数中,两个50×30 cm和50×40 cm的狭窄间距之间没有统计差异。回归分析显示,AUSiPC和AUDPC分别下降了13.07和12.66,单位间距的增加确保了模型的良好拟合。一个三变量等高线图说明了辣椒中矢量粉虱的丰度如何刺激ChLCD发生率并降低了辣椒产量,而3-D线框指出ChLCD发生率同时促进了AUSiPC和AUDPC。PCA分析确定了影响疾病发生率和辣椒产量的研究参数的主要成分。聚类分析很好地支持了PCA分析,并将所有研究的参数清楚地分为两个,一个与辣椒的增产特性相关,另一个与产量受限的ChLCD流行病学属性位于变量相似矩阵不同的节点上。

更新日期:2020-11-13
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