Burkholderia cepacia complex (Bcc) members have clinical relevance as opportunistic pathogens in patients with cystic fibrosis and are responsible of numerous nosocomial infections. These closely related bacteria are also reported as frequent contaminants of industrial products. In this retrospective study, we use PCR and recA gene sequence analysis to identify at species level Bcc isolates recovered from massive consumption products and industrial processes in Argentina during the last 25 years. The sequences obtained were also compared with recA sequences from clinical Bcc isolates deposited in GenBank database. We detected Bcc in purified water and preserved products from pharmaceutics, cosmetics, household cleaning articles, and beverages industries. B. contaminans (which is prevalent among people with cystic fibrosis in Argentina) was the most frequent Bcc species identified (42% of the Bcc isolates studied). B. cepacia (10%), B. cenocepacia (5%), B. vietnamiensis (16%), B. arboris (3%), and the recently defined B. aenigmatica (24%) were also detected. Rec A sequences from all B. cepacia and most B. contaminans industrial isolates obtained in this study displayed 100% identity with recA sequences from isolates infecting Argentinean patients. This information brings evidence for considering industrial massive consumption products as a potential source of Bcc infections. In addition, identification at species level in industrial microbiological laboratories is necessary for a better epidemiological surveillance. Particularly in Argentina, more studies are required in order to reveal the role of these products in the acquisition of B. contaminans infections.

洋葱伯克霍尔德菌复合体 (Bcc) 成员作为囊性纤维化患者的机会性病原体具有临床相关性，并且是许多医院感染的原因。这些密切相关的细菌也被报道为工业产品的常见污染物。在这项回顾性研究中，我们使用 PCR 和rec A 基因序列分析在物种水平上鉴定了过去 25 年阿根廷从大量消费产品和工业过程中回收的 Bcc 分离株。获得的序列还与来自存放在 GenBank 数据库中的临床 Bcc 分离株的rec A 序列进行了比较。我们在制药、化妆品、家居清洁用品和饮料行业的纯化水和防腐产品中检测到 Bcc。B. 污染（在阿根廷囊性纤维化患者中很普遍）是最常见的 Bcc 物种（研究的 Bcc 分离株的 42%）。还检测到 B. cepacia (10%)、B. cenocepacia (5%)、B. vietnamiensis (16%)、B. arboris (3%) 和最近定义的B. aenigmatica (24%)。本研究中获得的所有洋葱芽孢杆菌和大多数污染芽孢杆菌工业分离株的Rec A 序列与rec显示 100% 同一性来自感染阿根廷患者的分离株的序列。这些信息为将工业大规模消费产品视为 Bcc 感染的潜在来源提供了证据。此外，工业微生物实验室的物种水平鉴定对于更好的流行病学监测是必要的。特别是在阿根廷，需要更多的研究来揭示这些产品在感染双歧杆菌中的作用。