The oleaginous Jatropha curcas has been proposed as a promising source for biodiesel production in seed or potentially by in vitro production in cell cultures. However, little is known concerning the optimal growth conditions and the transcription of key factors and enzymes involved in the biosynthesis of storage lipids in J. curcas cell cultures. Additional knowledge is also needed for these factors in seeds. Here, we assess target gene expression in endosperm cells in planta and endosperm-derived cell suspension cultures (EDCCs). Endosperm cells were taken from three representative seed developmental stages, and cell suspensions were grown from these samples. Glucose, nitrogen, and abscisic acid concentrations were varied in an attempt to optimize biomass growth and oil yield. Oil production in EDCCs reached a maximum of 5% (w/w) of total lipids. Although much lower than lipid production in seed, lipid profiles of EDCCs remain identical to those produced in planta. The expression levels of five major transcription factors (TFs), as well as KAS1, accA, DGAT1/2 and PDAT1 enzymes, and the OLE1 protein, all key components of the lipid biosynthesis pathway were also measured. Significant expression of LEC1, FUS3, ABI3, and WRI1 was found in endosperm cells throughout seed development, suggesting similar functions to their counterparts in Arabidopsis and providing a reference expression level for cell cultures. J. curcas EDCCs showed lower expression of most TFs compared with endosperm tissue, with the exception of WRI1 which had comparable expression levels in the two systems. Conversely, the enzymes KAS1, accA, and DGAT had the same or higher expression levels in EDCCs versus endosperm cells. Interestingly, the genes that encoded for DGAT1 and DGAT2 enzymes were found preferentially expressed in endosperm cells and EDCCs, respectively. Contrary to other studies, our findings indicate that the addition of ABA does not result in increased expression of genes involved in storage lipid biosynthesis.

已经提出油性麻疯树是种子中生物柴油生产或细胞培养物中体外生产的有希望的来源。然而，关于最佳生长条件以及在J. curcas细胞培养物中与存储脂质的生物合成有关的关键因子和酶的转录知之甚少。种子中的这些因素还需要其他知识。在这里，我们评估了植物胚乳细胞中的靶基因表达和胚乳来源的细胞悬浮培养物（EDCC）。胚乳细胞取自三个代表性的种子发育阶段，并从这些样品中培养出细胞悬液。尝试改变葡萄糖，氮气和脱落酸的浓度，以优化生物量的生长和油的产量。EDCC中的产油量最多达到总脂质的5％（w / w）。尽管远低于种子中的脂质产量，但EDCC的脂质谱仍然与植物中产生的脂质谱相同。五个主要转录因子（TF）的表达水平以及KAS1，accA，DGAT1 / 2和PDAT1酶以及O LE1的表达水平蛋白质，脂质生物合成途径的所有关键组成部分也进行了测量。在整个种子发育过程中，胚乳细胞中发现了LEC1，FUS3，ABI3和WRI1的大量表达，这表明其功能与拟南芥中的对应物相似，并为细胞培养提供了参考表达水平。麻疯树EDCCs显示出与胚乳组织相比最TF的低表达，以下除外WRI1其具有在两个系统相媲美的表达水平。相反，酶KAS1，accA和DGAT在EDCC中具有相同或更高的表达水平与胚乳细胞 有趣的是，发现编码DGAT1和DGAT2酶的基因分别在胚乳细胞和EDCC中优先表达。与其他研究相反，我们的研究结果表明，ABA的添加不会导致参与储脂生物合成的基因表达增加。