This study investigated the effect of xylanase-assisted alkaline pretreatment (XAP) on the extraction of cellulose nanocrystal (CNCs) from energy cane (EC). Different concentrations of xylanase were tested to estimate the optimal enzyme loading required for pretreatment. XAP reduced the bleaching step from 7 to 5 h, generating a material with 93% whiteness index and a higher crystallinity index (CrI) than the alkaline-bleached fibers. A combined pretreatment using xylanase at 200 U/g removed 59 and 35% of hemicellulose and lignin, respectively, that was present in the EC which was confirmed using Fourier transform infrared spectroscopy and scanning electron microscopy analysis. X-ray diffraction and transmission electron microscopy revealed the XAP material required a shorter time of acid hydrolysis (30 min) to generate CNCs with similar lengths and CrI values to those obtained using only the alkaline pretreatment. Therefore, the XAP offered an efficient and promising approach for the isolation of CNCs with less use of chemicals for the bleaching process and a shorter hydrolysis time.

这项研究调查了木聚糖酶辅助碱预处理（XAP）对从能量棒（EC）提取纤维素纳米晶体（CNCs）的影响。测试了不同浓度的木聚糖酶，以估计预处理所需的最佳酶负载量。XAP将漂白步骤从7小时减少到5小时，生成的白度指数比碱性漂白纤维高93％，结晶度（CrI）高。使用木聚糖酶以200 U / g进行的联合预处理分别去除了EC中存在的59％和35％的半纤维素和木质素，这通过傅里叶变换红外光谱法和扫描电子显微镜分析得到了证实。X射线衍射和透射电子显微镜显示XAP材料需要较短的酸水解时间（30分钟）才能生成与仅使用碱性预处理获得的CNC具有相似的长度和CrI值的CNC。因此，XAP提供了一种有效且有前途的方法来分离CNC化合物，同时在漂白过程中使用的化学品更少，水解时间更短。