The practice of ethnomedicine remains to be the primary source of healthcare in many parts of the world, especially among the tribal communities. However, there is a lack of scientific outlook and investigation to authenticate and validate their medicinal values. The present study investigated the trace and heavy metal content, bioactive compounds, α-amylase, and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum using in vitro and in silico methods. Trace and heavy metal content of Rauvolfia tetraphylla and Oroxylum indicum were detected using Atomic Absorption Spectroscopy. Bioactive compounds were analyzed and identified by the GC-MS technique. α-Amylase and α-glucosidase inhibitory activity of the plants were studied using the spectrophotometric method using UV/VIS-Spectrophotometer. In silico molecular docking was carried out in AutoDock vina and the structures visualized using PyMol and Biovia Discovery Studio software. Statistical and graphical representations were performed using Excel and OriginPro. The trace and heavy metallic content such as Zn, Ni, Pb, Cr, Cu, and Mn were reported from both the plant. No Cd was detected in both the plants. GC-MS analysis revealed four major compounds in R. tetraphylla and seven in O. indicum. Biochemical studies showed that the leaf extract of O. indicum posses the strongest α-amylase and α-glucosidase inhibitory activity. R. tetraphylla showed weaker enzyme inhibition. Molecular docking study revealed that three compounds from O. indicum (O2, O3, and O6) and two from R. tetraphylla (R1 and R2) showed strong binding affinity to α-amylase and α-glucosidase. However, leaf extract of O. indicum showed better binding affinity with the enzymes compared to R. tetraphylla. Inhibition of α-amylase and α-glucosidase in an important strategy of diabetes control. The present study revealed the in vitro α-amylase and α-glucosidase inhibitory activity of Rauvolfia tetraphylla and Oroxylum indicum. In conclusion, the study identified that the leaf extract of O. indicum as a potential inhibitor of glucose metabolizing enzymes and could be a source of antidiabetic agents.

中文翻译：

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通过GC-MS鉴定四叶金叶紫菜和印度小叶紫菜的α-淀粉酶和α-葡萄糖苷酶抑制活性的体外和计算机模拟方法

在世界许多地区，特别是在部落社区中，族裔医学的实践仍然是医疗保健的主要来源。然而，缺乏鉴定和验证其药用价值的科学前景和研究。本研究使用体外和计算机模拟方法研究了四叶红景天和印度准绳的痕量和重金属含量，生物活性化合物，α-淀粉酶和α-葡萄糖苷酶的抑制活性。使用原子吸收光谱法检测四叶金叶菊和靛蓝的痕量和重金属含量。通过GC-MS技术分析和鉴定生物活性化合物。使用UV / VIS-分光光度计，通过分光光度法研究了植物的α-淀粉酶和α-葡萄糖苷酶抑制活性。在AutoDock静脉内进行计算机分子对接，并使用PyMol和Biovia Discovery Studio软件可视化结构。使用Excel和OriginPro进行统计和图形表示。两家工厂均报告了痕量和重金属含量，如锌，镍，铅，铬，铜和锰。在两个植物中均未检测到镉。GC-MS分析显示，四叶红豆草中有四种主要化合物，印度靛蓝中有七种主要化合物。生化研究表明，印度梧桐的叶提取物具有最强的α-淀粉酶和α-葡萄糖苷酶抑制活性。四叶红景天显示出较弱的酶抑制作用。分子对接研究表明，印度梧桐中的三种化合物（O2，O3和O6）和四叶猴中的两种化合物（R1和R2）对α-淀粉酶和α-葡萄糖苷酶具有很强的结合亲和力。但是，O的叶提取物。与四叶红僵菌相比，印度dic显示出与酶更好的结合亲和力。抑制α-淀粉酶和α-葡萄糖苷酶是控制糖尿病的重要策略。本研究揭示了四叶红景天和印度大头香的体外α-淀粉酶和α-葡萄糖苷酶抑制活性。总而言之，该研究确定了印度梧桐的叶提取物可以作为葡萄糖代谢酶的潜在抑制剂，并且可能是抗糖尿病药的来源。