In this study, a novel series of 2,4-dioxochroman-1,2,3-triazole hybrids 8a-l was synthesized by click reaction. These compounds were screened against α-glucosidase through in vitro and in silico evaluations. All the synthesized hybrids exhibited excellent α-glucosidase inhibition in comparison to standard drug acarbose. Representatively, 3-((((1-(3,4-dichlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methylene)chroman-2,4- dione 8h with IC50 = 20.1 ± 1.5 μM against α-glucosidase, was 37-times more potent than acarbose. Enzyme kinetic study revealed that compound 8h was a competitive inhibitor against α-glucosidase. In silico docking study on chloro derivatives 8h, 8g, and 8i were also performed in the active site of α -glucosidase. Evaluations on obtained interaction modes and binding energies of these compounds confirmed the results obtained through in vitro α-glucosidase inhibition.

在这项研究中，通过点击反应合成了一系列新的2,4-二氧杂色满-1,2,3-三唑杂化物8a-1。通过体外和计算机评估，筛选了针对α-葡萄糖苷酶的这些化合物。与标准药物阿卡波糖相比，所有合成的杂种均表现出优异的α-葡萄糖苷酶抑制作用。代表性地，3-（（（（（1-（3,4-二氯苄基）-1H-1,2,3-三唑-4-基）甲基）氨基）亚甲基）chroman-2,4-二酮8h，IC50 = 20.1抗α-葡萄糖苷酶的±1.5μM的效力是阿卡波糖的37倍。酶动力学研究表明，化合物8h是抗α-葡萄糖苷酶的竞争性抑制剂。在计算机对接研究中，还在α-葡萄糖苷酶的活性位点上对氯衍生物8h，8g和8i进行了研究。