A transgenic acute promyelocytic leukemia (APL) murine model established by Michael Bishop by cloning a human PML-RARα cDNA into the hMRP8 expression cassette has been widely used in the all-trans retinoid acid and arsenic preparations for the research of APL. However, in the existing literature, the data of regularity and characteristics of the pathogenesis of this model were still missing, which hinder the development of many studies, especially application of new technologies such as single-cell sequencing. Therefore, in this article, we have made up this part of the missing data using an improved APL murine model. We clarified the effects of different inoculation doses on the onset time, latency, morbidity, life span, and proportion of APL cells in peripheral blood (PB), spleen, bone marrow, and so on. The relationship between the proportion of APL cells in the bone marrow, spleen, and PB and organ histological changes was also revealed. These results were a supplement and refinement of this APL model. It would add to the knowledge base of the field and aid in ensuring that accurate models are used for directed interventions. It also provides a great convenience for the researchers who will carry out similar research.

中文翻译：

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分享与帮助：一种广泛使用的转基因引发的小鼠急性早幼粒细胞白血病模型的发病规律和特点

Michael Bishop 通过将人PML-RARα cDNA 克隆到hMRP8 中建立的转基因急性早幼粒细胞白血病 (APL) 鼠模型表达盒已广泛用于APL研究的全反式维甲酸和砷制剂。然而，现有文献中仍缺乏该模型发病规律和特征的数据，阻碍了许多研究的开展，尤其是单细胞测序等新技术的应用。因此，在本文中，我们使用改进的 APL 鼠模型弥补了这部分缺失数据。我们阐明了不同接种剂量对外周血 (PB)、脾脏、骨髓等 APL 细胞的起效时间、潜伏期、发病率、寿命和比例的影响。还揭示了骨髓、脾脏和PB中APL细胞的比例与器官组织学变化之间的关系。这些结果是对该 APL 模型的补充和完善。它将增加该领域的知识库，并有助于确保使用准确的模型进行定向干预。也为以后进行类似研究的研究人员提供了极大的便利。