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Changes in ATP sulfurylase activity in response to altered cyanobacteria growth conditions.
bioRxiv - Physiology Pub Date : 2020-11-12 , DOI: 10.1101/2020.11.11.378158
Lucia Gastoldi , Lewis M. Ward , Mayuko Nakagawa , Mario Giordano , Shawn E. McGlynn

Here we investigated variations in cell growth and ATP sulfurylase activity when two cyanobacterial strains - Synechocystis sp. PCC6803 and Synechococcus sp. WH7803 - were grown comparatively between conventional media and media with low ammonium, low sulfate and a controlled high CO2/low O2 atmosphere, which might resemble some Precambrian environments. In both organisms, a transition and adaptation to the reconstructed environmental media resulted in a decrease in ATPS specific activity. This decrease in activity appears to be decoupled from growth rate, suggesting the enzyme is not rate-limiting in S assimilation and raising questions about the role of ATPS redox regulation in cell physiology and thorughout history.

中文翻译:

响应于改变的蓝细菌生长条件,ATP硫酸化酶活性的变化。

在这里,我们研究了当两个蓝细菌菌株-Synechocystis sp。时细胞生长和ATP硫化酶活性的变化。PCC6803和Synechococcus sp。WH7803-在常规培养基和低铵,低硫酸盐和受控的高CO2 /低O2气氛的培养基之间进行比较生长,这可能类似于某些前寒武纪环境。在这两种生物中,对重建环境培养基的过渡和适应导致ATPS比活性的降低。活性的降低似乎与生长速率脱钩,表明该酶在S同化中没有速率限制,并引发了有关ATPS氧化还原调节在细胞生理学和药物史中的作用的疑问。
更新日期:2020-11-12
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