Sugarcane brown rust, caused by Puccinia melanocephala, is one of the main diseases of sugarcane in China. The identification and discovery of new resistance genes have important theoretical and practical significance for preventing outbreaks of brown rust and ensuring the sustainable production of sugarcane. To screen for polymorphic simple-sequence repeat (SSR) molecular markers for localization of brown rust resistance genes, we used two populations that are suitable for genetic linkage map construction and mapping of new resistance genes to construct resistant and susceptible genetic pools. We then screened 449 pairs of primers to identify polymorphic SSR markers in the parental lines and the resistant/susceptible genetic pools. The results showed that 25 pairs of primers directed amplification of polymorphic DNA fragments between the parents of the cross combination ‘Yuetang 03-393’ × ‘ROC 24’, and 16 pairs of primers amplified polymorphic fragments between the parents of the cross combination ‘Liucheng 03-1137’ × ‘Dezhe 93-88’. Four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636 and SCESSR2551) amplified polymorphic DNA fragments between the parental lines and the resistant/susceptible genetic pools in ‘Yuetang 03-393’ × ‘ROC 24’. The results of this study will establish a solid foundation for the mapping of new brown rust resistance genes, genetic linkage map construction and the development of closely-associated molecular markers in sugarcane.

中文翻译：

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在抗性和易感基因库中检测与新的甘蔗褐锈病抗性基因连锁的多态性单序列重复标记

甘蔗褐锈病，由黑头锈菌，是我国甘蔗的主要病害之一。新抗性基因的鉴定和发现对于防止褐锈病的爆发和保障甘蔗的可持续生产具有重要的理论和现实意义。为了筛选用于定位褐锈病抗性基因的多态性简单序列重复（SSR）分子标记，我们使用了两个适合遗传连锁图构建和新抗性基因作图的群体来构建抗性和易感遗传库。然后，我们筛选了 449 对引物以鉴定亲本系和抗性/易感遗传库中的多态性 SSR 标记。结果表明，25对引物定向扩增杂交组合‘悦堂03-393’בROC 24’亲本间的多态性DNA片段，16对引物扩增出杂交组合'柳城03-1137'×'得哲93-88'亲本间的多态性片段。四对引物（SMC236CG、SCESSR0928、SCESSR0636 和 SCESSR2551）扩增了‘月堂 03-393’בROC 24’中亲本系和抗/易感遗传库之间的多态性 DNA 片段。本研究结果将为甘蔗抗褐锈病新基因的定位、遗传连锁图谱的构建和密切相关分子标记的开发奠定坚实的基础。SCESSR0636 和 SCESSR2551) 在‘月堂 03-393’בROC 24’中扩增了亲本系和抗性/易感遗传库之间的多态性 DNA 片段。本研究结果将为甘蔗抗褐锈病新基因的定位、遗传连锁图谱的构建和密切相关分子标记的开发奠定坚实的基础。SCESSR0636 和 SCESSR2551) 在‘月堂 03-393’בROC 24’中扩增了亲本系和抗性/易感遗传库之间的多态性 DNA 片段。本研究结果将为甘蔗抗褐锈病新基因的定位、遗传连锁图谱的构建和密切相关分子标记的开发奠定坚实的基础。