Abstract

Objective

To investigate the regulatory effect of long non-coding RNA (lncRNA) SUMO1P3 on invasion, migration and cell cycle of gastric cancer (GC) cells through Wnt/β-catenin signaling pathway.

Methods

Tumor tissues and adjacent normal tissues from the GC patients were collected, and human normal gastric epithelial cells GES1 and GC cells SGC-7901, MKN45, HGC-27 and AGS were selected for study. The expression of SUMO1P3 in GC tissues and cells were detected by RT-qPCR. The effects of SUMO1P3 on the proliferation, invasion and migration of SGC-7901 and MKN45 cells were detected by CCK-8, transwell and wound healing assay respectively, and the effects of SUMO1P3 on apoptosis and cycle progression of SGC-7901 and MKN45 cells were detected by flow cytometry. The expressions of Wnt/β-catenin pathway-related and cell cycle-related proteins were detected by Western blot.

Results

The expression of SUMO1P3 was significantly upregulated in GC tissues and cell lines. Downregulation of SUMO1P3 significantly inhibited the SGC-7901 and MKN45 cell proliferation, invasion, migration, and cycle progression and promoted the cell apoptosis, while overexpression of SUMO1P3 showed the opposite effect. Further study showed that downregulation of SUMO1P3 significantly reduced the expressions of Wnt1, β-catenin, c-myc, and Cyclin D1 in SGC-7901 and MKN45 cells.

Conclusion

SUMO1P3 may promote invasion, migration, and cycle progression of SGC-7901 and MKN45 cells by enhancing the Wnt/β-catenin pathway.

中文翻译：

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LncRNA SUMO1P3通过Wnt/β-catenin信号通路调控胃癌细胞的侵袭、迁移和细胞周期

摘要

客观的

探讨长链非编码RNA（lncRNA）SUMO1P3通过Wnt/β-catenin信号通路对胃癌（GC）细胞侵袭、迁移和细胞周期的调控作用。

方法

收集GC患者的肿瘤组织和邻近的正常组织，选择人正常胃上皮细胞GES1和GC细胞SGC-7901、MKN45、HGC-27和AGS进行研究。RT-qPCR 检测 SUMO1P3 在 GC 组织和细胞中的表达。分别采用CCK-8、transwell和创面愈合试验检测SUMO1P3对SGC-7901和MKN45细胞增殖、侵袭和迁移的影响，SUMO1P3对SGC-7901和MKN45细胞凋亡和周期进程的影响分别为流式细胞仪检测。Western blot检测Wnt/β-catenin通路相关蛋白和细胞周期相关蛋白的表达。

结果

SUMO1P3的表达在GC组织和细胞系中显着上调。SUMO1P3的下调显着抑制SGC-7901和MKN45细胞的增殖、侵袭、迁移和周期进程，促进细胞凋亡，而SUMO1P3的过表达则表现出相反的作用。进一步的研究表明，SUMO1P3 的下调显着降低了 SGC-7901 和 MKN45 细胞中 Wnt1、β-catenin、c-myc 和 Cyclin D1 的表达。

结论

SUMO1P3 可能通过增强 Wnt/β-catenin 通路促进 SGC-7901 和 MKN45 细胞的侵袭、迁移和周期进程。