Abstract

Subsurface lineages of microorganisms are often questioned for their autochthonous nature. We hypothesize that sporulation is critical to the delivery and ubiquity of Halanaerobium congolense in global subsurface hydrocarbon reservoirs, forming spores, or spore-like structures in order to survive surface conditions, and thriving in subsurface reservoir conditions. Genomic analysis of H. congolense revealed the presence of various genes essential for sporulation, many of which are homologous to those involved in various stages of sporulation in clostridia and bacilli. Transcriptomics revealed transcription of sporulation genes in H. congolense grown in sub-optimal conditions. This is further supported by microscopy evidence of spore-like structures.

摘要

微生物的地下世系经常因其自身的自然特性而受到质疑。我们假设孢子形成对于在地下地下碳氢化合物储层中结缕藻的运输和普遍存在至关重要，形成孢子或类似孢子的结构以在地表条件下生存并在地下储层条件下蓬勃发展。贡氏嗜血杆菌的基因组分析揭示了孢子形成所必需的各种基因的存在，其中许多与梭菌和杆菌中孢子形成的各个阶段所涉及的那些基因同源。转录组学揭示了在次优条件下生长的Congolense中孢子形成基因的转录。显微镜下的孢子状结构证据进一步证明了这一点。