The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10‐year‐old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.

中文翻译：

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药理性射精从精囊炎的种马中收集的精液的特征

本研究比较了人工阴道或药理学上从10岁的纯种马与精囊炎引起的射精所收集的精子质量。药理学方案包括静脉内施用托咪定（0.01 mg / kg）和催产素（20 IU），并在所有精液收集尝试中成功诱导射精。评估精子活力，质膜和顶体完整性（PMAI），活性氧（ROS）水平，多形核中性粒细胞（PMN）百分比以及新鲜和冷却精液（5°C持续24小时）的细菌分布。通过药理学方法获得的精液精液减少，精子活力降低，精子活力增强。而且，通过药理学方法收集的精液中观察到较高的PMAI和较低的ROS水平。