In genetic fish breeding research, clear pedigree information is of great significance for breeding and parental management. In order to establish a stable, highly accurate, and widely applicable parentage identification method for tilapia, 13 highly polymorphic microsatellite loci within populations of Oreochromis niloticus, O. aureus, O. niloticus × O. aureus, and their mixed population were screened. Four groups of fluorescent‐labeled multiple capillary electrophoresis were established for allelic genotyping. The assignment success rate reached 100% when 7, 9, 8 and 12 loci were used in the population of O. niloticus, O. aureus, O. niloticus × O. aureus, and their mixed population, respectively. All 175 progeny individuals of “Yuemin No. 1” tilapia were exclusively assigned to their parental pairs when the 12 loci for the mixed population were used. This study established a fluorescent‐labeled microsatellite‐based parentage assignment method for O. niloticus, O. aureus, O. niloticus × O. aureus, and their mixed population with high identification accuracy and efficiency, which lays a foundation for pedigree information and population genetic management in tilapia breeding.

中文翻译：

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基于微卫星的亲缘关系鉴定方法用于尼罗罗非鱼，金黄色葡萄球菌和尼罗罗非鱼×金黄色葡萄球菌的鉴定

在遗传鱼类育种研究中，清晰的家谱信息对育种和父母管理具有重要意义。为了建立稳定的，高度精确的，而且广泛地适用亲子鉴定方法对于罗非鱼，群内13高度多态性微卫星基因座尼罗罗非鱼，ö。金黄色，O。尼罗罗非斯 ×  O。筛选了金黄色葡萄球菌及其混合种群。建立了四组荧光标记的多重毛细管电泳用于等位基因基因分型。当在O. niloticus，O. aureus种群中使用7、9、8和12个基因座时，分配成功率达到100％，O。niloticus×O. aureus及其混合种群。当使用混合种群的12个基因座时，“ 175号岳敏”罗非鱼的所有175个后代个体都被单独分配给其父母对。这项研究建立了一种荧光标记的基于微卫星的亲本分配方法，用于识别O. niloticus，O。aureus，O。niloticus×O. aureus及其混合种群，具有较高的识别准确性和效率，为谱系信息和种群奠定了基础。罗非鱼育种的遗传管理。