Silver (Ag)/silver chloride (AgCl) nanoparticles have been used worldwide for their antimicrobial activity. Proteases play an important role in many physiological processes during wound healing. Therefore, the aim of this study was to fabricate silver-type nanoparticles exhibiting protease activity for medical applications such as wound healing and dressings. The Ag/AgCl nanoparticles were fabricated using Bacillus sp. protease and visible light activation. The size of the fabricated nanoparticles was estimated to be 35.29 ± 6.43 nm. The nanoparticles were coated on a cotton gauze bandage using immersion and ultrasonication. Scanning electron microscopy and energy dispersive X-ray spectroscopy confirmed that the nanoparticles could be used to coat the gauze bandage. Synchrotron radiation X-ray tomographic microscopy indicated that coating with the nanoparticles did not destroy the packing of cotton fibers in the gauze bandage. The nanoparticles exhibited fibrinolytic and collagenolytic activities. Protease activity remained after the nanoparticle coating was applied to the gauze bandage. The nanoparticles were not absorbed on a gelatin agar plate after incubation at 37 °C for 18 H. These results suggest that the coated cotton gauze bandage may be safe for further use, and the nanoparticles may not be absorbed into animal or human skin.

中文翻译：

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芽孢杆菌的应用。蛋白酶在溶液和棉纱绷带中制备银/氯化银纳米粒子

银 (Ag)/氯化银 (AgCl) 纳米粒子因其抗菌活性已在世界范围内使用。蛋白酶在伤口愈合过程中的许多生理过程中发挥重要作用。因此，本研究的目的是制造具有蛋白酶活性的银型纳米粒子，用于伤口愈合和敷料等医学应用。使用芽孢杆菌制造 Ag/AgCl 纳米粒子sp。蛋白酶和可见光活化。制造的纳米粒子的尺寸估计为 35.29 ± 6.43 nm。使用浸泡和超声处理将纳米颗粒涂覆在棉纱布绷带上。扫描电子显微镜和能量色散 X 射线光谱证实纳米粒子可用于包覆纱布绷带。同步辐射 X 射线断层显微术表明，用纳米粒子涂层不会破坏纱布绷带中棉纤维的堆积。纳米颗粒表现出纤维蛋白溶解和胶原溶解活性。在将纳米颗粒涂层应用于纱布绷带后，蛋白酶活性仍然存在。在 37°C 下孵育 18 小时后，纳米颗粒未吸附在明胶琼脂板上。