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Phytosynthesis of biohybrid nano-silver anchors enhanced size dependent photocatalytic, antibacterial, anticancer properties and cytocompatibility
Process Biochemistry ( IF 3.7 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.procbio.2020.11.008
S. Priyadarshini , Faridah Sonsudin , Azizah Mainal , Rosiyah Yahya , V. Gopinath , Jamuna Vadivelu , Khaloud Mohammed Alarjani , Dunia A. Al Farraj , Hany Mohamed Yehia

Abstract Metallic silver tactically attains reductant from biomaterials during biosynthesis, though it is still challenging to fasten the rate of stable nano-silver/silver chloride particles (NAg/AgClPs) synthesis. In this study, two different sizes of NAg/AgClPs (15.9 and 24.2 nm) were synthesised using Cissus quadrangularis stem (CQS) extract under microwave irradiation. The synthesised NAg/AgClPs were characterised using high throughput traditional methods. The antibacterial study of these materials demonstrated excellent activity against human nosocomial pathogens. The metabolic activity and viability assay provided strong evidence that the 15.9 nm NAg/AgClPs inhibited the bacterial pathogens better than the 24.2 nm NAg/AgClPs. The cytotoxicity of the as-synthesised NAg/AgClPs was studied towards human A549 adenocarcinomic alveolar basal epithelial cell line and RAW 264.7 macrophage cells. Anticancer activity of both 15.9 nm NAg/AgClPs and 24.2 nm NAg/AgClPs displayed the IC50 values at 85 μg/mL and 90 μg/mL against A549 cells while RAW 264.7 macrophage cells expressed the IC50 values at 130 μg/mL and 140 μg/mL respectively. Further, the synthesised NAg/AgClPs demonstrated high photocatalytic activity towards degradation of environmental pollutant methylene blue (MB) under visible light illumination. Our results suggested that the synthesised NAg/AgClPs can be used to develop novel antimicrobial, anticancer formulations and environmental catalyst.

中文翻译:

生物杂化纳米银锚的植物合成增强了尺寸依赖性光催化、抗菌、抗癌特性和细胞相容性

摘要 金属银在生物合成过程中从生物材料中获得还原剂,但加快稳定纳米银/氯化银颗粒 (NAg/AgClPs) 合成的速度仍然具有挑战性。在这项研究中,在微波辐射下,使用 Cissus quadrangularis 茎 (CQS) 提取物合成了两种不同大小的 NAg/AgClP(15.9 和 24.2 nm)。合成的 NAg/AgClP 使用高通量传统方法进行表征。这些材料的抗菌研究证明了对人类医院病原体的优异活性。代谢活性和活力测定提供了强有力的证据,证明 15.9 nm NAg/AgClPs 比 24.2 nm NAg/AgClPs 更好地抑制细菌病原体。研究了合成的 NAg/AgClPs 对人 A549 腺癌肺泡基底上皮细胞系和 RAW 264.7 巨噬细胞的细胞毒性。15.9 nm NAg/AgClPs 和 24.2 nm NAg/AgClPs 的抗癌活性在 85 μg/mL 和 90 μg/mL 时对 A549 细胞显示出 IC50 值,而 RAW 264.7 巨噬细胞在 130 μg/mL 和 140 μg/mL 时表达 IC50 值毫升分别。此外,合成的 NAg/AgClPs 在可见光照射下对环境污染物亚甲蓝 (MB) 的降解表现出很高的光催化活性。我们的结果表明合成的 NAg/AgClPs 可用于开发新型抗菌、抗癌制剂和环境催化剂。2 nm NAg/AgClPs 在 85 μg/mL 和 90 μg/mL 时显示了对 A549 细胞的 IC50 值,而 RAW 264.7 巨噬细胞分别在 130 μg/mL 和 140 μg/mL 时表达了 IC50 值。此外,合成的 NAg/AgClPs 在可见光照射下对环境污染物亚甲蓝 (MB) 的降解表现出很高的光催化活性。我们的结果表明合成的 NAg/AgClPs 可用于开发新型抗菌、抗癌制剂和环境催化剂。2 nm NAg/AgClPs 在 85 μg/mL 和 90 μg/mL 时显示了对 A549 细胞的 IC50 值,而 RAW 264.7 巨噬细胞分别在 130 μg/mL 和 140 μg/mL 时表达了 IC50 值。此外,合成的 NAg/AgClPs 在可见光照射下对环境污染物亚甲蓝 (MB) 的降解表现出很高的光催化活性。我们的结果表明合成的 NAg/AgClPs 可用于开发新型抗菌、抗癌制剂和环境催化剂。
更新日期:2021-02-01
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