Abstract Nano hydroxyapatite (nHA) was synthesized from different methods. Spherical-like shaped nanoparticles of nHA were synthesized by chemical precipitation using Euclea Natalensis root extract as a template for the first time. In addition, traditional synthesis was performed, obtaining rod-like nanoparticles. The formation of nHA was confirmed using Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), and Transmission Electron Microscopy (TEM). HA from green route presented a spherical-like shape with smooth surface. However, nanogrooves covered over the surface of nHA obtained without green template. Additionally, the cytotoxic effect of the nHA nanoparticles on L929 viability was assessed using MTS assay. The particles exhibited less cellular toxicity at shorter incubation periods. Spherical-like and rod-like nHA particles interact differently with fibroblast after 24 h of evaluation.

中文翻译：

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纳米羟基磷灰石的绿色合成：形态变化及其对成纤维细胞细胞毒性的影响

摘要 纳米羟基磷灰石（nHA）是由不同的方法合成的。首次以桉树根提取物为模板，通过化学沉淀法合成了类似球形的 nHA 纳米颗粒。此外，进行传统合成，获得棒状纳米粒子。使用傅里叶变换红外 (FTIR)、X 射线衍射 (XRD) 和透射电子显微镜 (TEM) 确认了 nHA 的形成。绿色路线的HA呈球形，表面光滑。然而，纳米槽覆盖在没有绿色模板的情况下获得的 nHA 表面。此外，使用 MTS 测定评估了 nHA 纳米颗粒对 L929 活力的细胞毒性作用。这些颗粒在较短的孵育时间内表现出较低的细胞毒性。