Aims

This study is to investigate the role of adenovirus type 36 (Ad36) in inducing differentiation of human adipose-derived stem cells (hADSCs) into brown adipocytes.

Main methods

The hADSCs were induced to differentiate into adipocytes by a cocktail method and Ad36, respectively. They were collected on the 2nd, 4th, 6th, and 8th day, respectively. LncRNA ROR was silenced by siRNA. RT-qPCR and Western-blot were used to detect the mRNA and protein levels. Transmission electron microscopy was used to observe the mitochondria.

Key findings

The mRNA and protein expression levels of LncRNA ROR, Cidea, Dio2, Fgf21, Ucp1, Prdm16, Cox5b, Atp5o, Atp6, and Nd2 in the Ad36 induction group were significantly higher than those in the cocktail induction group (P < 0.05). The expression levels of Leptin mRNA and protein in the Ad36 induction group were significantly lower than those in the cocktail induction group (P < 0.01). After siRNA knockdown of LncRNA ROR, mRNA and protein expression levels of Cidea, Dio2, Fgf21, Ucp1, Prdm16, Cox5b, Atp5o, Atp6 and Nd2 were significantly lower than the control group during the induction of hADSC differentiation into adipocytes by Ad36 (P < 0.05). Additionally, mitochondria in the Ad36 induction group was increased compared to that in the cocktail induction group.

Significance

Ad36 may promote the differentiation of hADSCs into brown adipocytes by up-regulating LncRNA ROR.

中文翻译：

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Ad36通过上调LncRNA ROR促进hADSCs分化为棕色脂肪细胞

目的

这项研究旨在调查36型腺病毒（Ad36）在诱导人脂肪来源的干细胞（hADSCs）分化为褐色脂肪细胞中的作用。

主要方法

分别通过鸡尾酒法和Ad36诱导hADSCs分化为脂肪细胞。它们分别在第2、4、6和8天收集。LncRNA ROR被siRNA沉默。用RT-qPCR和Western-blot检测mRNA和蛋白质水平。用透射电子显微镜观察线粒体。

主要发现

Ad36诱导组LncRNA ROR，Cidea，Dio2，Fgf21，Ucp1，Prdm16，Cox5b，Atp50o，Atp6和Nd2的mRNA和蛋白表达水平明显高于鸡尾酒诱导组（P  <0.05）。Ad36诱导组瘦素mRNA和蛋白的表达水平明显低于鸡尾酒诱导组（P  <0.01）。siRNA敲低LncRNA ROR后，Cidea的mRNA和蛋白表达水平Ad36诱导hADSC向脂肪细胞分化的过程中，Dio2，Fgf21，Ucp1，Prdm16，Cox5b，Atp50o，Atp6和Nd2明显低于对照组（P  <0.05）。另外，与鸡尾酒诱导组相比，Ad36诱导组的线粒体增加。

意义

Ad36可能通过上调LncRNA ROR促进hADSCs向褐色脂肪细胞的分化。