Biometals ( IF 4.1 ) Pub Date : 2020-11-12 , DOI: 10.1007/s10534-020-00267-9 Karla Vega-Granados 1 , Juan Cruz-Reyes 1 , José F Horta-Marrón 1 , Manuel Marí-Beffa 2 , Laura Díaz-Rubio 1 , Iván Córdova-Guerrero 1 , Daniel Chávez-Velasco 3 , M Carmen Ocaña 4 , Miguel A Medina 4 , Lilian B Romero-Sánchez 1
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Abstract
Octyltrimethylammonium tetrathiotungstate salt (ATT-C8) was synthesized and its ability to chelate copper was evaluated. The biological and toxic aspects were evaluated by in vitro and in vivo assays, using bovine aorta endothelial cells (BAEC) and zebrafish (Danio rerio) embryos. The obtained results suggest that ATT-C8 has better biocompatibility, showing a significantly lower lethal concentration 50 (LC50) value in comparison to ammonium tetrathiotungstate (ATT). Zebrafish embryos assay results indicate that both tetrathiotungstate salts at the studied concentrations increase the hatching time. Even more, an in vivo assay showed that synthesized materials behave as copper antagonists and have the ability to inhibit its toxicological effects. Also, both materials were found to be active for the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The characterization of the materials was carried out using the following spectroscopic techniques: Ultraviolet–Visible (UV–Vis), Fourier Transform Infrared (FTIR) and proton nuclear magnetic resonance (1H-NRM).
Graphic abstract
中文翻译：
四硫钨酸辛基三甲基铵的合成、表征和生物学评价
摘要
合成了四硫钨酸辛基三甲基铵盐 (ATT-C8) 并评估了其螯合铜的能力。使用牛主动脉内皮细胞 (BAEC) 和斑马鱼 ( Danio rerio ) 胚胎，通过体外和体内试验对生物学和毒性方面进行了评估。所得结果表明 ATT-C8 具有更好的生物相容性，显示出显着较低的致死浓度 50 (LC 50) 值与四硫钨酸铵 (ATT) 相比。斑马鱼胚胎测定结果表明，研究浓度的两种四硫钨酸盐都会增加孵化时间。更重要的是，体内试验表明合成材料具有铜拮抗剂的作用，并具有抑制其毒理学作用的能力。此外，发现这两种材料对体外 2,2-二苯基-1-苦基肼 (DPPH) 测定具有活性。使用以下光谱技术对材料进行表征：紫外-可见（UV-Vis）、傅里叶变换红外（FTIR）和质子核磁共振（1 H-NRM）。
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