Hyperoxia at resuscitation increases oxidative stress, and even brief exposure to high oxygen concentrations during stabilization may trigger organ injury with adverse long-term outcomes in premature infants. We studied the long-term effects of short-term perinatal oxygen exposure on cell cycle gene expression and lung growth in adult mice. We randomized mice litters at birth to 21, 40, or 100%O2 for 30 min and recovered in room air for 4 or 12 weeks. Cell cycle gene expression, protein analysis, and lung morphometry were assessed at 4 and 12 weeks. The principal component analysis demonstrated a high degree of correlation for cell cycle gene expression among the three oxygen groups. Lung elastin was significantly lower in the 100%O2 groups at 4 weeks. On lung morphometry, radial alveolar count, alveolar number, and septal count were similar. However, the mean linear intercept (MLI) and septal length significantly correlated among the oxygen groups. The MLI was markedly higher in the 100%O2 groups at 4 and 12 weeks of age, and the septal length was significantly lower in the 100%O2 groups at 12 weeks. Short-term exposure to high oxygen concentrations lead to subtle changes in lung development that may affect alveolarization. The changes are related explicitly to secondary crest formation that may result in alteration in lung elastin. Resuscitation with high oxygen concentrations may have a significant impact on lung development and long-term outcomes such as BPD in premature infants.

中文翻译：

---

围产期短期接触氧气可能会损害成年小鼠的肺发育

复苏时的高氧血症会增加氧化应激，甚至在稳定过程中短暂暴露于高氧浓度下也可能触发器官损伤，对早产儿造成不良的长期后果。我们研究了短期围产期氧气暴露对成年小鼠细胞周期基因表达和肺生长的长期影响。我们在出生时将小鼠幼仔随机分为21、40或100％O2，持续30分钟，然后在室内空气中恢复4或12周。在第4周和第12周评估细胞周期基因表达，蛋白质分析和肺形态。主成分分析表明三个氧基团之间的细胞周期基因表达高度相关。100％O2组的肺弹性蛋白在第4周显着降低。在肺形态学上，放射状肺泡计数，肺泡计数和间隔计数相似。但是，平均线性截距（MLI）和间隔长度在氧基团之间显着相关。100％O2组在4周和12周龄时的MLI明显较高，而100％O2组在12周时的间隔长度显着降低。短期暴露于高浓度的氧气会导致肺部发育的细微变化，从而可能影响肺泡形成。这些变化与继发性rest形成明显相关，继发性rest形成可能导致肺弹性蛋白改变。高氧浓度的复苏可能对早产儿的肺发育和长期结局（例如BPD）产生重大影响。在第12周时，100％O2组的间隔长度显着降低。短期暴露于高浓度的氧气会导致肺部发育的细微变化，从而可能影响肺泡形成。这些变化与继发性rest形成明显相关，继发性rest形成可能导致肺弹性蛋白改变。高氧浓度的复苏可能对早产儿的肺发育和长期结局（例如BPD）产生重大影响。在第12周时，100％O2组的间隔长度显着降低。短期暴露于高浓度的氧气会导致肺部发育的细微变化，从而可能影响肺泡形成。这些变化与继发性rest形成明显相关，继发性rest形成可能导致肺弹性蛋白改变。高氧浓度的复苏可能对早产儿的肺发育和长期结局（例如BPD）产生重大影响。