The toxicity of ethanedinitrile (EDN), an alternative fumigant for stored products, is decreased due to the high sorption of the gas in treated commodities. This laboratory study examined adding carbon dioxide to EDN to increase activity against Rhyzopertha dominica and Lasioderma serricorne at 25 °C, 50–70% R.H and a photoperiod of 16 L:8D h for 24 h. CO₂ at 30% with the estimated LC₂₅ dose of EDN exhibited a significant effect on the mortality of adults of R. dominica and the larvae, pupae, and adults of L. serricorne. Adults emerged from the mixed life stage cultures of R. dominica exposed to a mixture of 75 ppm or 150 ppm of EDN and CO₂ at 10, 20 and 30% averaged 26.8, 11.5 and 4.9, and 1.5, 0.6 and 0.9, respectively. Averages of 188.6 and 56.6 adults resulted from cultures of R. dominica fumigated with EDN at 75 and 150 ppm alone, respectively. The L. serricorne cultures exposed to EDN at 500 or 1000 ppm plus 10, 20, and 30% of CO₂ yielded average numbers of emerged adults of 170.5, 110.9 and 114.6, and 68.3, 30.2 and 31.9, respectively, compared to 358.3 and 173.6 in cultures treated with 500 and 1000 ppm of EDN alone, respectively. Numbers of adults of both species emerged from cultures exposed to either EDN or CO₂ alone in the presence of commodities were significantly higher than those exposed to the EDN + CO₂ (30%) mixture. These results clearly indicated the positive effect of CO₂ on toxicity of EDN that can overcome the decrease of EDN toxicity against the treated insects due to its sorption in commodities.

乙二腈 (EDN) 是一种用于储存产品的替代熏蒸剂，其毒性由于气体在处理过的商品中的高吸附性而降低。该实验室研究检查了在 25 °C、50–70% RH 和 16 L:8D h 的光周期下，向 EDN 添加二氧化碳以增加对多米尼卡丝虫和丝角线虫的活性，持续 24 小时。30% 的CO ₂和估计的 LC ₂₅剂量的 EDN 对R. dominica的成虫和L. serricorne的幼虫、蛹和成虫的死亡率有显着影响。成虫从暴露于 75 ppm 或 150 ppm EDN 和 CO ₂的混合物中的多米尼克红藻混合生命阶段培养物中出现在 10%、20% 和 30% 时，平均分别为 26.8、11.5 和 4.9，以及 1.5、0.6 和 0.9。分别用 75 ppm 和 150 ppm 的 EDN 熏蒸的多米尼克红毛虫培养物平均产生 188.6 只和 56.6 只成虫。与 500 或 1000 ppm 的 EDN 以及 10、20 和 30% 的 CO ₂接触的 L. serricorne培养物产生的成虫平均数量分别为 170.5、110.9 和 114.6 以及 68.3、30.2 和 31.9，而分别为 35.3 和 35.35。在分别用 500 和 1000 ppm 的 EDN 单独处理的培养物中为 173.6。在商品存在的情况下，从单独暴露于 EDN 或 CO _{2 的}培养物中出现的两种物种的成虫数量显着高于暴露于 EDN + CO _{2 的那些}(30%) 混合物。这些结果清楚地表明 CO ₂对 EDN 的毒性有积极影响，可以克服 EDN 对处理过的昆虫的毒性由于其在商品中的吸附而降低的问题。